Publication: Development of an improved reverse genetics system for Akabane bunyavirus
Issued Date
2016-06-01
Resource Type
ISSN
18790984
01660934
01660934
Other identifier(s)
2-s2.0-84960153721
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Mahidol University
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SCOPUS
Bibliographic Citation
Journal of Virological Methods. Vol.232, (2016), 16-20
Suggested Citation
Akiko Takenaka-Uema, Keita Sugiura, Norasuthi Bangphoomi, Chieko Shioda, Kazuyuki Uchida, Kentaro Kato, Takeshi Haga, Shin Murakami, Hiroomi Akashi, Taisuke Horimoto Development of an improved reverse genetics system for Akabane bunyavirus. Journal of Virological Methods. Vol.232, (2016), 16-20. doi:10.1016/j.jviromet.2015.12.014 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/40803
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Title
Development of an improved reverse genetics system for Akabane bunyavirus
Abstract
© 2016 Elsevier B.V. Akabane disease, caused by the insect-transmitted Akabane virus (AKAV), affects livestock by causing life-threatening deformities or mortality of fetuses. Therefore, Akabane disease has led to notable economic losses in numerous countries, including Japan. In this short communication, a new T7 RNA polymerase-based AKAV reverse genetics system was developed. Using this system, in which three plasmids transcribing antigenomic RNAs were transfected into cells stably expressing T7 polymerase, we successfully reconstituted the live attenuated vaccine TS-C2 strain (named rTTT), and also generated a mutant AKAV (rTTTδNSs) that lacked the gene encoding the nonstructural NSs protein, which is regarded as a virulence factor. Analysis of growth kinetics revealed that rTTTδNSs grew at a much slower rate than the rTTT and TS-C2 virus. These results suggest that our established reverse genetics system is a powerful tool that can be used for AKAV vaccine studies with gene-manipulated viruses.