Publication: Monte Carlo simulation of the effects of vesicle geometry on calcium microdomains and neurotransmitter release
Issued Date
2016-07-01
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ISSN
12860050
12860042
12860042
Other identifier(s)
2-s2.0-84979911719
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Mahidol University
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SCOPUS
Bibliographic Citation
EPJ Applied Physics. Vol.75, No.1 (2016)
Suggested Citation
Praopim Limsakul, Charin Modchang Monte Carlo simulation of the effects of vesicle geometry on calcium microdomains and neurotransmitter release. EPJ Applied Physics. Vol.75, No.1 (2016). doi:10.1051/epjap/2016150299 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/40939
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Title
Monte Carlo simulation of the effects of vesicle geometry on calcium microdomains and neurotransmitter release
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Abstract
© EDP Sciences, 2016. We investigate the effects of synaptic vesicle geometry on Ca2+ diffusion dynamics in presynaptic terminals using MCell, a realistic Monte Carlo algorithm that tracks individual molecules. By modeling the vesicle as a sphere and an oblate or a prolate spheroid with a reflective boundary, we measure the Ca2+ concentration at various positions relative to the vesicle. We find that the presence of a vesicle as a diffusion barrier modifies the shape of the [Ca2+] microdomain in the vicinity of the vesicle. Ca2+ diffusion dynamics also depend on the distance between the vesicle and the voltage-gated calcium channels (VGCCs) and on the shape of the vesicle. The oblate spheroidal vesicle increases the [Ca2+] up to six times higher than that in the absence of a vesicle, while the prolate spheroidal vesicle can increase the [Ca2+] only 1.4 times. Our results also show that the presence of vesicles that have different geometries can maximally influence the [Ca2+] microdomain when the vesicle is located less than 50 nm from VGCCs.