Publication: Tricomponent immunopotentiating system as a novel molecular design strategy for malaria vaccine development
1
Issued Date
2011-10-01
Resource Type
ISSN
10985522
00199567
00199567
Other identifier(s)
2-s2.0-80855136511
Rights
Mahidol University
Rights Holder(s)
SCOPUS
Bibliographic Citation
Infection and Immunity. Vol.79, No.10 (2011), 4260-4275
Suggested Citation
Takeshi Miyata, Tetsuya Harakuni, Takafumi Tsuboi, Jetsumon Sattabongkot, Ayumu Ikehara, Mayumi Tachibana, Motomi Torii, Goro Matsuzaki, Takeshi Arakawa Tricomponent immunopotentiating system as a novel molecular design strategy for malaria vaccine development. Infection and Immunity. Vol.79, No.10 (2011), 4260-4275. doi:10.1128/IAI.05214-11 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/11988
Research Projects
Organizational Units
Authors
Journal Issue
Thesis
Title
Tricomponent immunopotentiating system as a novel molecular design strategy for malaria vaccine development
Abstract
The creation of subunit vaccines to prevent malaria infection has been hampered by the intrinsically weak immunogenicity of the recombinant antigens. We have developed a novel strategy to increase immune responses by creating genetic fusion proteins to target specific antigen-presenting cells (APCs). The fusion complex was composed of three physically linked molecular entities: (i) a vaccine antigen, (ii) a multimeric α-helical coiled-coil core, and (iii) an APC-targeting ligand linked to the core via a flexible linker. The vaccine efficacy of the tricomponent complex was evaluated using an ookinete surface protein of Plasmodium vivax, Pvs25, and merozoite surface protein-1 of Plasmodium yoelii. Immunization of mice with the tricomponent complex induced a robust antibody response and conferred substantial levels of P. vivax transmission blockade as evaluated by a membrane feed assay, as well as protection from lethal P. yoelii infection. The observed effect was strongly dependent on the presence of all three components physically integrated as a fusion complex. This system, designated the tricomponent immunopotentiating system (TIPS), onto which any recombinant protein antigens or nonproteinaceous substances could be loaded, may be a promising strategy for devising subunit vaccines or adjuvants against various infectious diseases, including malaria. © 2011, American Society for Microbiology.