Publication: Dengue virus type 2 recognizes the carbohydrate moiety of neutral glycosphingolipids in mammalian and mosquito cells
Issued Date
2011-02-01
Resource Type
ISSN
13480421
03855600
03855600
Other identifier(s)
2-s2.0-79251506368
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Mahidol University
Rights Holder(s)
SCOPUS
Bibliographic Citation
Microbiology and Immunology. Vol.55, No.2 (2011), 135-140
Suggested Citation
Sineewanlaya Wichit, Akanitt Jittmittraphap, Kazuya I.P.J. Hidari, Butsaya Thaisomboonsuk, Songsak Petmitr, Sukathida Ubol, Chie Aoki, Saki Itonori, Koichi Morita, Takashi Suzuki, Yasuo Suzuki, Wipawee Jampangern Dengue virus type 2 recognizes the carbohydrate moiety of neutral glycosphingolipids in mammalian and mosquito cells. Microbiology and Immunology. Vol.55, No.2 (2011), 135-140. doi:10.1111/j.1348-0421.2010.00293.x Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/12087
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Title
Dengue virus type 2 recognizes the carbohydrate moiety of neutral glycosphingolipids in mammalian and mosquito cells
Abstract
Dengue viruses infect cells by attaching to a surface receptor which remains unknown. The putative receptor molecules of dengue virus type 2 on the surface of mosquito (AP-61) and mammalian (LLC-MK2) cell lines were investigated. The immunochemical detection and structural analysis of carbohydrates demonstrated that the neutral glycosphingolipids, L-3 (GlcNAcβ1-3Manβ1-4Glcβ1-1'Cer) in AP-61 cells, and nLc 4 Cer (Galβ1-4GlcNAcβ1-3Galβ1-4Glcβ1-1'Cer) in LLC-MK2 cells were recognized by the virus. These findings strongly suggest that neutral glycosphingolipids share the key determinant for virus binding and that the β-GlcNAc residue may play an important role in dengue virus binding to the host cell surface. © 2011 The Societies and Blackwell Publishing Asia Pty Ltd.