Publication: Marmosets (Callithrix jacchus) as a non-human primate model for evaluation of candidate dengue vaccines: Induction and maintenance of specific protective immunity against challenges with clinical isolates
Issued Date
2017-12-01
Resource Type
ISSN
14652099
00221317
00221317
Other identifier(s)
2-s2.0-85037721517
Rights
Mahidol University
Rights Holder(s)
SCOPUS
Bibliographic Citation
Journal of General Virology. Vol.98, No.12 (2017), 2955-2967
Suggested Citation
Meng Ling Moi, Yasushi Ami, Nor Azila Muhammad Azami, Kenji Shirai, Sutee Yoksan, Yuriko Suzaki, Kazutaka Kitaura, Chang Kweng Lim, Masayuki Saijo, Ryuji Suzuki, Tomohiko Takasaki, Ichiro Kurane Marmosets (Callithrix jacchus) as a non-human primate model for evaluation of candidate dengue vaccines: Induction and maintenance of specific protective immunity against challenges with clinical isolates. Journal of General Virology. Vol.98, No.12 (2017), 2955-2967. doi:10.1099/jgv.0.000913 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/42734
Research Projects
Organizational Units
Authors
Journal Issue
Thesis
Title
Marmosets (Callithrix jacchus) as a non-human primate model for evaluation of candidate dengue vaccines: Induction and maintenance of specific protective immunity against challenges with clinical isolates
Abstract
© 2017 The Authors. Dengue virus (DENV) is one of the major infectious diseases in tropical regions and approximately half of the world population is at risk of infection. Vaccines would offer an effective control measure against this disease. We previously reported on the utility of marmosets as an animal model for studying primary and secondary dengue infections. Infected marmosets consistently develop viraemia and antibody kinetics that reflect those of patients with dengue. Thus, it is important to determine the utility of marmosets as an animal model for demonstrating vaccine efficacy. In this study, marmosets were inoculated with candidate vaccine and parent strains and challenged with a clinical DENV strain. The viraemia and antibody kinetics in these marmosets were determined. Marmosets consistently develop lower viraemia with an attenuated vaccine strain. During secondary challenge, the IgM response was delayed, whereas the IgG levels rose rapidly, indicating a secondary antibody response. The neutralizing activities against the homotypic serotype were high; all marmosets were protected against viraemia following secondary inoculation. The viraemia markers and antibody responses were consistent with those of human DENV infection and vaccinees. These results demonstrate the utility of marmosets as an animal model for the study of vaccine efficacy.