Publication: Development of Nested PCR-Heteroduplex Mobility Assay for Determination of Genetic Diversity in the Block 2 Region of the Plasmodium falciparum Merozoite Surface Protein 1 Gene
Issued Date
2020-01-01
Resource Type
ISSN
20900031
20900023
20900023
Other identifier(s)
2-s2.0-85084078248
Rights
Mahidol University
Rights Holder(s)
SCOPUS
Bibliographic Citation
Journal of Parasitology Research. Vol.2020, (2020)
Suggested Citation
Kanyanan Kritsiriwuthinan, Warunee Ngrenngarmlert, Sakone Sunantaraporn, Anna Jehmah Development of Nested PCR-Heteroduplex Mobility Assay for Determination of Genetic Diversity in the Block 2 Region of the Plasmodium falciparum Merozoite Surface Protein 1 Gene. Journal of Parasitology Research. Vol.2020, (2020). doi:10.1155/2020/9520326 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/56218
Research Projects
Organizational Units
Authors
Journal Issue
Thesis
Title
Development of Nested PCR-Heteroduplex Mobility Assay for Determination of Genetic Diversity in the Block 2 Region of the Plasmodium falciparum Merozoite Surface Protein 1 Gene
Other Contributor(s)
Abstract
© 2020 Kanyanan Kritsiriwuthinan et al. Genetic diversity of Plasmodium parasite has significantly related to malaria control and vaccine development. The P. falciparum merozoite surface protein 1 (Pfmsp1) gene is a commonly used molecular marker to differentiate genetic diversity. This study is aimed at developing a nested PCR-Heteroduplex Mobility Assay (nPCR-HMA) for determination of the block 2 of the Pfmsp1 gene. The MAD20 family allele of P. falciparum was used as a control for optimization of the annealing and polyacrylamide gel electrophoresis conditions. In order to evaluate the developed nPCR-HMA, 8 clinical P. falciparum isolates were examined for allelic variants. The results revealed 9 allelic variants. Our study indicated that the successful nPCR-HMA with good precision and accuracy offers a more rapid, efficient, and cheap method for large-scale molecular epidemiological studies as compared to nucleotide sequencing.