Publication: Slow turning lateral vessel bioreactor improves embryoid body formation and cardiogenic differentiation of mouse embryonic stem cells
Issued Date
2013-10-01
Resource Type
ISSN
21524998
21524971
21524971
Other identifier(s)
2-s2.0-84885008764
Rights
Mahidol University
Rights Holder(s)
SCOPUS
Bibliographic Citation
Cellular Reprogramming. Vol.15, No.5 (2013), 443-458
Suggested Citation
Sasitorn Rungarunlert, Nuttha Klincumhom, Theerawat Tharasanit, Mongkol Techakumphu, Melinda K. Pirity, Andras Dinnyes Slow turning lateral vessel bioreactor improves embryoid body formation and cardiogenic differentiation of mouse embryonic stem cells. Cellular Reprogramming. Vol.15, No.5 (2013), 443-458. doi:10.1089/cell.2012.0082 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/31207
Research Projects
Organizational Units
Authors
Journal Issue
Thesis
Title
Slow turning lateral vessel bioreactor improves embryoid body formation and cardiogenic differentiation of mouse embryonic stem cells
Abstract
Embryonic stem cells (ESCs) have the ability to form aggregates, which are called embryoid bodies (EBs). EBs mimic early embryonic development and are commonly produced for cardiomyogenesis. Here, we describe a method of EB formation in hydrodynamic conditions using a slow-turning lateral vessel (STLV) bioreactor and the subsequent differentiation of EBs into cardiomyocytes. EBs formed in the STLV were compared with conventional techniques, such as hanging drop (HD) or static suspension cell culture (SSC), for homogeneity of EB size, shape, proliferation, apoptosis, and in vitro cardiac differentiation. After 3 days of culture, a four-fold improvement in the yield of EB formation/mL, a six-fold enhancement in total yield of EB/mL, and a nearly 10-fold reduction of cells that failed to incorporate into EBs were achieved in STLV versus SSC. During cardiac differentiation, a 1.5- to 4.2-fold increase in the area of cardiac troponin T (cTnT) per single EB in STLV versus SSC and HD was achieved. These results demonstrate that the STLV method improves the quality and quantity of ES cells to form EBs and enhances the efficiency of cardiac differentiation. We have demonstrated that the mechanical method of cell differentiation creates different microenvironments for the cells and thus influences their lineage commitments, even when genetic origin and the culture medium are the same. Ascorbic acid (ASC) improved further cardiac commitment in differentiation assays. Hence, this culture system is suitable for the production of large numbers of cells for clinical cell replacement therapies and industrial drug testing applications. © Mary Ann Liebert, Inc.