Publication: Novel Analytical Platform For Robust Identification of Cell Migration Inhibitors
Issued Date
2020-12-01
Resource Type
ISSN
20452322
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2-s2.0-85078168908
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Mahidol University
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SCOPUS
Bibliographic Citation
Scientific Reports. Vol.10, No.1 (2020)
Suggested Citation
Parinyachat Somchai, Kriengkrai Phongkitkarun, Patipark Kueanjinda, Supawan Jamnongsong, Kulthida Vaeteewoottacharn, Vor Luvira, Seiji Okada, Siwanon Jirawatnotai, Somponnat Sampattavanich Novel Analytical Platform For Robust Identification of Cell Migration Inhibitors. Scientific Reports. Vol.10, No.1 (2020). doi:10.1038/s41598-020-57806-0 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/53934
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Title
Novel Analytical Platform For Robust Identification of Cell Migration Inhibitors
Abstract
© 2020, The Author(s). Wound healing assay is a simple and cost-effective in vitro assay for assessing therapeutic impacts on cell migration. Its key limitation is the possible confoundment by other cellular phenotypes, causing misinterpretation of the experimental outcome. In this study, we attempted to address this problem by developing a simple analytical approach for scoring therapeutic influences on both cell migration and cell death, while normalizing the influence of cell growth using Mitomycin C pre-treatment. By carefully mapping the relationship between cell death and wound closure rate, contribution of cell death and cell migration on the observed wound closure delay can be quantitatively separated at all drug dosing. We showed that both intrinsic cell motility difference and extrinsic factors such as cell seeding density can significantly affect final interpretation of therapeutic impacts on cellular phenotypes. Such discrepancy can be rectified by using the actual wound closure time of each treatment condition for the calculation of phenotypic scores. Finally, we demonstrated a screen for strong pharmaceutical inhibitors of cell migration in cholangiocarcinoma cell lines. Our approach enables accurate scoring of both migrastatic and cytotoxic effects, and can be easily implemented for high-throughput drug screening.