Publication: A haemagglutination test for rapid detection of antibodies to SARS-CoV-2
Issued Date
2021-12-01
Resource Type
ISSN
20411723
Other identifier(s)
2-s2.0-85103541293
Rights
Mahidol University
Rights Holder(s)
SCOPUS
Bibliographic Citation
Nature Communications. Vol.12, No.1 (2021)
Suggested Citation
Alain Townsend, Pramila Rijal, Julie Xiao, Tiong Kit Tan, Kuan Ying A. Huang, Lisa Schimanski, Jiandong Huo, Nimesh Gupta, Rolle Rahikainen, Philippa C. Matthews, Derrick Crook, Sarah Hoosdally, Susanna Dunachie, Eleanor Barnes, Teresa Street, Christopher P. Conlon, John Frater, Carolina V. Arancibia-Cárcamo, Justine Rudkin, Nicole Stoesser, Fredrik Karpe, Matthew Neville, Rutger Ploeg, Marta Oliveira, David J. Roberts, Abigail A. Lamikanra, Hoi Pat Tsang, Abbie Bown, Richard Vipond, Alexander J. Mentzer, Julian C. Knight, Andrew J. Kwok, Gavin R. Screaton, Juthathip Mongkolsapaya, Wanwisa Dejnirattisai, Piyada Supasa, Paul Klenerman, Christina Dold, J. Kenneth Baillie, Shona C. Moore, Peter J.M. Openshaw, Malcolm G. Semple, Lance C.W. Turtle, Mark Ainsworth, Alice Allcock, Sally Beer, Sagida Bibi, Donal Skelly, Lizzy Stafford, Katie Jeffrey, Denise O’Donnell, Elizabeth Clutterbuck, Alexis Espinosa, Maria Mendoza, Dominique Georgiou, Teresa Lockett, Jose Martinez, Elena Perez, Veronica Gallardo Sanchez, Giuseppe Scozzafava, Alberto Sobrinodiaz, Hannah Thraves, Etienne Joly A haemagglutination test for rapid detection of antibodies to SARS-CoV-2. Nature Communications. Vol.12, No.1 (2021). doi:10.1038/s41467-021-22045-y Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/75942
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Title
A haemagglutination test for rapid detection of antibodies to SARS-CoV-2
Author(s)
Alain Townsend
Pramila Rijal
Julie Xiao
Tiong Kit Tan
Kuan Ying A. Huang
Lisa Schimanski
Jiandong Huo
Nimesh Gupta
Rolle Rahikainen
Philippa C. Matthews
Derrick Crook
Sarah Hoosdally
Susanna Dunachie
Eleanor Barnes
Teresa Street
Christopher P. Conlon
John Frater
Carolina V. Arancibia-Cárcamo
Justine Rudkin
Nicole Stoesser
Fredrik Karpe
Matthew Neville
Rutger Ploeg
Marta Oliveira
David J. Roberts
Abigail A. Lamikanra
Hoi Pat Tsang
Abbie Bown
Richard Vipond
Alexander J. Mentzer
Julian C. Knight
Andrew J. Kwok
Gavin R. Screaton
Juthathip Mongkolsapaya
Wanwisa Dejnirattisai
Piyada Supasa
Paul Klenerman
Christina Dold
J. Kenneth Baillie
Shona C. Moore
Peter J.M. Openshaw
Malcolm G. Semple
Lance C.W. Turtle
Mark Ainsworth
Alice Allcock
Sally Beer
Sagida Bibi
Donal Skelly
Lizzy Stafford
Katie Jeffrey
Denise O’Donnell
Elizabeth Clutterbuck
Alexis Espinosa
Maria Mendoza
Dominique Georgiou
Teresa Lockett
Jose Martinez
Elena Perez
Veronica Gallardo Sanchez
Giuseppe Scozzafava
Alberto Sobrinodiaz
Hannah Thraves
Etienne Joly
Pramila Rijal
Julie Xiao
Tiong Kit Tan
Kuan Ying A. Huang
Lisa Schimanski
Jiandong Huo
Nimesh Gupta
Rolle Rahikainen
Philippa C. Matthews
Derrick Crook
Sarah Hoosdally
Susanna Dunachie
Eleanor Barnes
Teresa Street
Christopher P. Conlon
John Frater
Carolina V. Arancibia-Cárcamo
Justine Rudkin
Nicole Stoesser
Fredrik Karpe
Matthew Neville
Rutger Ploeg
Marta Oliveira
David J. Roberts
Abigail A. Lamikanra
Hoi Pat Tsang
Abbie Bown
Richard Vipond
Alexander J. Mentzer
Julian C. Knight
Andrew J. Kwok
Gavin R. Screaton
Juthathip Mongkolsapaya
Wanwisa Dejnirattisai
Piyada Supasa
Paul Klenerman
Christina Dold
J. Kenneth Baillie
Shona C. Moore
Peter J.M. Openshaw
Malcolm G. Semple
Lance C.W. Turtle
Mark Ainsworth
Alice Allcock
Sally Beer
Sagida Bibi
Donal Skelly
Lizzy Stafford
Katie Jeffrey
Denise O’Donnell
Elizabeth Clutterbuck
Alexis Espinosa
Maria Mendoza
Dominique Georgiou
Teresa Lockett
Jose Martinez
Elena Perez
Veronica Gallardo Sanchez
Giuseppe Scozzafava
Alberto Sobrinodiaz
Hannah Thraves
Etienne Joly
Other Contributor(s)
Siriraj Hospital
Oxford University Hospitals NHS Foundation Trust
Public Health England
Université Fédérale Toulouse Midi-Pyrénées
The Wellcome Centre for Human Genetics
Chang Gung University College of Medicine
Chang Gung Memorial Hospital
University of Oxford
University of Edinburgh, Roslin Institute
University of Liverpool
National Institute of Immunology India
National Heart and Lung Institute
Nuffield Department of Medicine
John Radcliffe Hospital
University of Oxford Medical Sciences Division
Rosalind Franklin Institute
Oxford University Hospitals NHS Foundation Trust
Public Health England
Université Fédérale Toulouse Midi-Pyrénées
The Wellcome Centre for Human Genetics
Chang Gung University College of Medicine
Chang Gung Memorial Hospital
University of Oxford
University of Edinburgh, Roslin Institute
University of Liverpool
National Institute of Immunology India
National Heart and Lung Institute
Nuffield Department of Medicine
John Radcliffe Hospital
University of Oxford Medical Sciences Division
Rosalind Franklin Institute
Abstract
Serological detection of antibodies to SARS-CoV-2 is essential for establishing rates of seroconversion in populations, and for seeking evidence for a level of antibody that may be protective against COVID-19 disease. Several high-performance commercial tests have been described, but these require centralised laboratory facilities that are comparatively expensive, and therefore not available universally. Red cell agglutination tests do not require special equipment, are read by eye, have short development times, low cost and can be applied at the Point of Care. Here we describe a quantitative Haemagglutination test (HAT) for the detection of antibodies to the receptor binding domain of the SARS-CoV-2 spike protein. The HAT has a sensitivity of 90% and specificity of 99% for detection of antibodies after a PCR diagnosed infection. We will supply aliquots of the test reagent sufficient for ten thousand test wells free of charge to qualified research groups anywhere in the world.