Publication: Xanthones from mangosteen inhibit inflammation in human macrophages and in human adipocytes exposed to macrophage-conditioned media
Issued Date
2010-04-01
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ISSN
15416100
00223166
00223166
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2-s2.0-77949755216
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Mahidol University
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SCOPUS
Bibliographic Citation
Journal of Nutrition. Vol.140, No.4 (2010), 842-847
Suggested Citation
Akkarach Bumrungpert, Ruchaneekorn W. Kalpravidh, Chia Chi Chuang, Angel Overman, Kristina Martinez, Arion Kennedy, Michael McIntosh Xanthones from mangosteen inhibit inflammation in human macrophages and in human adipocytes exposed to macrophage-conditioned media. Journal of Nutrition. Vol.140, No.4 (2010), 842-847. doi:10.3945/jn.109.120022 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/29723
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Title
Xanthones from mangosteen inhibit inflammation in human macrophages and in human adipocytes exposed to macrophage-conditioned media
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Abstract
Obesity-associated inflammation is characterized by recruitment of macrophages (MΦ) into white adipose tissue (WAT) and production of inflammatory cytokines, leading to the development of insulin resistance. The xanthones, α- and γ-mangostin (MG), are major bioactive compounds found in mangosteen that are reported to have antiinflammatory and antioxidant properties. Thus, we examined the efficacy of MG to prevent lipopolysaccharide (LPS)-mediated inflammation in human MΦ (differentiated U937 cells) and cross-talk with primary cultures of newly differentiated human adipocytes. We found that α- and γ-MG attenuated LPS-induced expression of inflammatory genes, including tumor necrosis factor-α, interleukin-6, and interferon γ-inducible protein-10 in a dose-dependent manner in MΦ. We also found that α- and γ-MG attenuated LPS-activated mitogen-activated protein kinases (MAPK) and activator protein (AP)-1, but only γ-MG reduced nuclear factor-κB (NF-κB). In addition, α- and γ-MG attenuated LPS suppression of PPARγ gene expression in a dose-dependent manner. Notably, the ability of MΦ-conditioned media to cause inflammation and insulin resistance in primary cultures of human adipocytes was attenuated by pretreating MΦ with γ-MG. Taken together, these data demonstrate that MG attenuates LPS-mediated inflammation in MF and insulin resistance in adipocytes, possibly by preventing the activation of MAPK, NF-κB, and AP-1, which are central to inflammatory cytokine production in WAT. © 2010 American Society for Nutrition.