Publication: Evaluation of SHP1-P2 methylation as a biomarker of lymph node metastasis in patients with squamous cell carcinoma of the head and neck
Issued Date
2019-09-20
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ISSN
1875855X
19057415
19057415
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2-s2.0-85072702480
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Mahidol University
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SCOPUS
Bibliographic Citation
Asian Biomedicine. Vol.12, No.3 (2019), 111-116
Suggested Citation
Nakarin Kitkumthorn, Somboon Keelawat, Jutamas Wongphoom, Prakasit Rattanatanyong, Apiwat Mutirangura Evaluation of SHP1-P2 methylation as a biomarker of lymph node metastasis in patients with squamous cell carcinoma of the head and neck. Asian Biomedicine. Vol.12, No.3 (2019), 111-116. doi:10.1515/abm-2019-0009 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/50078
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Title
Evaluation of SHP1-P2 methylation as a biomarker of lymph node metastasis in patients with squamous cell carcinoma of the head and neck
Abstract
© 2018Nakarin Kitkumthorn et al., published by Sciendo. Hypermethylation of Src homology region 2 domain-containing protein-tyrosine phosphatase 1 promoter 2 (SHP1-P2) has been proven as an epithelial-specific marker. This marker has been used for the detection of lymph node metastasis in patients with lung cancer or colon cancer. To investigate SHP1-P2 methylation in patients with squamous cell carcinoma of the head and neck (HNSCC) and determine its potential for micrometastasis detection in the lymph nodes of patients with HNSCC. SHP1-P2 methylation levels were analyzed by combined methylation-specific primer TaqMan real-time PCR in 5 sample groups: normal tonsils (n = 10), microdissected squamous cell carcinoma epithelia (n = 9), nonmetastatic head and neck cancer lymph nodes (LN N0, n = 15), metastatic HNSCC histologically negative for tumor cells (LN-, n = 18), and matched cases histologically positive for tumor cells (LN+, n = 18). SHP1-P2 methylation of 10.27 ± 4.05% was found in normal tonsils as a lymphoid tissue baseline, whereas it was 61.31 ± 17.00% in microdissected cancer cell controls. In the 3 lymph node groups, the SHP1-P2 methylation levels were 9.99 ± 6.61% for LN N0, 14.49 ± 10.03% for LN-Nx, and 41.01 ± 24.51% for LN+ Nx. The methylation levels for LN-Nx and LN+ Nx were significantly different (P = 0.0002). Receiver operating characteristic curve analysis of SHP1-P2 methylation demonstrated an area under the curve of 0.637 in distinguishing LN N0 from LN-Nx. SHP1-P2 methylation was high in HNSCC, and low in lymphoid tissues. This methylation difference is concordant with lymph node metastasis.