Publication: A proteasome inhibitor produced by Burkholderia pseudomallei modulates intracellular growth
Issued Date
2017-06-01
Resource Type
ISSN
10961208
08824010
08824010
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2-s2.0-85016434887
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Mahidol University
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SCOPUS
Bibliographic Citation
Microbial Pathogenesis. Vol.107, (2017), 175-180
Suggested Citation
Sariqa Wagley, Muthita Vanaporn, Darawan Rinchai, Laura Conejero, Ganjana Lertmemongkolchai, Gregory J. Bancroft, Richard W. Titball A proteasome inhibitor produced by Burkholderia pseudomallei modulates intracellular growth. Microbial Pathogenesis. Vol.107, (2017), 175-180. doi:10.1016/j.micpath.2017.03.015 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/42835
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Title
A proteasome inhibitor produced by Burkholderia pseudomallei modulates intracellular growth
Abstract
© 2017 Elsevier Ltd The NRPS/PKS cluster encodes the enzymes necessary for glidobactin synthesis it is partially conserved in various members of the Burkholderia genus including B. pseudomallei. In this study we have shown that the insertional inactivation or deletion of glbC in this cluster in B. pseudomallei could reduce the ability of the bacterium to survive or grow in murine macrophages or in human neutrophils. Exogenously added proteasome inhibitors were able to chemically complement the mutation. The insertional inactivation or deletion of glbC increased virulence in an acute model of infection in Balb/c or C57BL/6 mice but virulence in a chronic model of infection was similar to that of the wild type. Our findings contrast with the previous finding that inactivation of the glb gene cluster in B. pseudomallei strain 1026b resulted in marked attenuation, and provides evidence of differential roles for some genes in virulence of different strains of B. pseudomallei.