Publication:
Establishment of Intestinal Epithelial Cell Monolayers and Their Use in Calcium Switch Assay for Assessment of Intestinal Tight Junction Assembly

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dc.contributor.authorPawin Pongkorpsakolen_US
dc.contributor.authorWilasinee Satianrapapongen_US
dc.contributor.authorPreedajit Wongkrasanten_US
dc.contributor.authorPeter R. Steinhagenen_US
dc.contributor.authorNuttha Tuangkijkulen_US
dc.contributor.authorNutthapoom Pathomthongtaweechaien_US
dc.contributor.authorChatchai Muanprasaten_US
dc.contributor.otherRamathibodi Hospitalen_US
dc.contributor.otherCharité – Universitätsmedizin Berlinen_US
dc.contributor.otherChulabhorn Royal Academyen_US
dc.contributor.otherFaculty of Medicine Ramathibodi Hospital, Mahidol Universityen_US
dc.contributor.otherMahidol Universityen_US
dc.date.accessioned2022-08-04T08:14:04Z
dc.date.available2022-08-04T08:14:04Z
dc.date.issued2021-01-01en_US
dc.description.abstractIntestinal barrier function relies primarily on the assembly and integrity of tight junctions, which forms a size-selective barrier. This barrier restricts paracellular movement of solutes in various types of epithelia. Of note, extracellular Ca2+ concentration affects tight junction assembly. Therefore, the removal and re-addition of Ca2+ into cell culture medium of cultured intestinal epithelial cells causes destabilization and reassembly of tight junction to membrane periphery near apical surface, respectively. Based on this principle, the Ca2+-switch assay was established to investigate tight junction assembly in fully differentiated intestinal epithelial cells. This chapter provides a stepwise protocol for culture of intestinal epithelial cell monolayers using T84 cell line as an in vitro model and the Ca2+-switch assay for evaluating tight junction assembly.en_US
dc.identifier.citationMethods in Molecular Biology. Vol.2367, (2021), 273-290en_US
dc.identifier.doi10.1007/7651_2021_347en_US
dc.identifier.issn19406029en_US
dc.identifier.issn10643745en_US
dc.identifier.other2-s2.0-85109524305en_US
dc.identifier.urihttps://repository.li.mahidol.ac.th/handle/20.500.14594/76360
dc.rightsMahidol Universityen_US
dc.rights.holderSCOPUSen_US
dc.source.urihttps://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85109524305&origin=inwarden_US
dc.subjectBiochemistry, Genetics and Molecular Biologyen_US
dc.titleEstablishment of Intestinal Epithelial Cell Monolayers and Their Use in Calcium Switch Assay for Assessment of Intestinal Tight Junction Assemblyen_US
dc.typeBook Chapteren_US
dspace.entity.typePublication
mu.datasource.scopushttps://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=85109524305&origin=inwarden_US

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