Publication: The rpoE operon regulates heat stress response in Burkholderia pseudomallei
Issued Date
2008-07-01
Resource Type
ISSN
15746968
03781097
03781097
Other identifier(s)
2-s2.0-44949254948
Rights
Mahidol University
Rights Holder(s)
SCOPUS
Bibliographic Citation
FEMS Microbiology Letters. Vol.284, No.2 (2008), 191-196
Suggested Citation
Muthita Vanaporn, Paiboon Vattanaviboon, Visith Thongboonkerd, Sunee Korbsrisate The rpoE operon regulates heat stress response in Burkholderia pseudomallei. FEMS Microbiology Letters. Vol.284, No.2 (2008), 191-196. doi:10.1111/j.1574-6968.2008.01216.x Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/18900
Research Projects
Organizational Units
Authors
Journal Issue
Thesis
Title
The rpoE operon regulates heat stress response in Burkholderia pseudomallei
Other Contributor(s)
Abstract
Burkholderia pseudomallei is a gram-negative bacterium and the causative agent of melioidosis, one of the important lethal diseases in tropical regions. In this article, we demonstrate the crucial role of the B. pseudomallei rpoE locus in the response to heat stress. The rpoE operon knockout mutant exhibited growth retardation and reduced survival when exposed to a high temperature. Expression analysis using rpoH promoter-lacZ fusion revealed that heat stress induction of rpoH, which encodes heat shock sigma factor (σH), was abolished in the B. pseudomallei rpoE mutant. Analysis of the rpoH promoter region revealed sequences sharing high homology to the consensus sequence of σE-dependent promoters. Moreover, the putative heat-induced σH-regulated heat shock proteins (i.e. GroEL and HtpG) were also absent in the rpoE operon mutant. Altogether, our data suggest that the rpoE operon regulates B. pseudomallei heat stress response through the function of rpoH. © 2008 Federation of European Microbiological Societies. Published by Blackwell Publishing Ltd. All rights reserved.