Publication: Biased cyclical electrical field-flow fractionation for separation of submicron particles
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Issued Date
2016-01-01
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ISSN
16182650
16182642
16182642
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2-s2.0-84955175541
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Mahidol University
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SCOPUS
Bibliographic Citation
Analytical and Bioanalytical Chemistry. Vol.408, No.3 (2016), 855-863
Suggested Citation
Mathuros Ornthai, Atitaya Siripinyanond, Bruce K. Gale Biased cyclical electrical field-flow fractionation for separation of submicron particles. Analytical and Bioanalytical Chemistry. Vol.408, No.3 (2016), 855-863. doi:10.1007/s00216-015-9173-5 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/43219
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Title
Biased cyclical electrical field-flow fractionation for separation of submicron particles
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Abstract
© 2015 Springer-Verlag Berlin Heidelberg. The potential of biased cyclical electrical field-flow fractionation (BCyElFFF), which applies the positive cycle voltage longer than the negative cycle voltage, for characterization of submicron particles, was investigated. Parameters affecting separation and retention such as voltage, frequency, and duty cycle were examined. The results suggest that the separation mechanism in BCyElFFF in many cases is more related to the size of particles, as is the case with normal ElFFF, in the studied conditions, than the electrophoretic mobility, which is what the theory predicts for CyElFFF. However, better resolution was obtained when separating using BCyElFFF mode than when using normal CyElFFF. BCyElFFF was able to demonstrate simultaneous baseline separations of a mixture of 0.04-, 0.1-, and 0.2-μm particles and near separation of 0.5-μm particles. This study has shown the applicability of BCyElFFF for separation and characterization of submicron particles greater than 0.1-μm in size, which had not been demonstrated previously. The separation and retention results suggest that for particles of this size, retention is based more on particle size than on electrophoretic mobility, which is contrary to existing theory for CyElFFF.