Publication: Potentiation of carbon tetrachloride hepatotoxicity by piperine
Issued Date
1995-01-01
Resource Type
ISSN
01480545
Other identifier(s)
2-s2.0-0028865367
Rights
Mahidol University
Rights Holder(s)
SCOPUS
Bibliographic Citation
Drug and Chemical Toxicology. Vol.18, No.4 (1995), 333-344
Suggested Citation
P. Piyachaturawat, S. Kingkaeohoi, C. Toskulkao Potentiation of carbon tetrachloride hepatotoxicity by piperine. Drug and Chemical Toxicology. Vol.18, No.4 (1995), 333-344. doi:10.3109/01480549509014327 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/17267
Research Projects
Organizational Units
Authors
Journal Issue
Thesis
Title
Potentiation of carbon tetrachloride hepatotoxicity by piperine
Author(s)
Other Contributor(s)
Abstract
The effect of piperine on CCl4-induced hepatotoxicity was investigated in rats. Piperine pretreatment potentiated the hepatotoxicity of CCI4 in a dose-dependent manner. The maximum potentiation occurred when piperine at a dose of 100 mg/kg BW was intragastrically administered 4 h prior to an intraperitoneal injection of CCl4, at which time the activities of plasma glutamic pyruvic transaminase (PGPT) and plasma glutamic oxaloacetic transaminase (PGOT) were elevated by 70-80% Concurrent with the rise in PGPT and PGOT activities, the accumulation of hepatic triglyceride increased whereas the plasma level of triglyceride decreased. Piperine pretreatment also potentiated CCl4-induced lipid peroxidation in the liver. The extent of potentiation correlated well with the rise of hepatic enzyme activity in plasma. In the in vitro system in which the tissue was preincubated with piperine and CCI4 was added into the incubation medium, piperine also exhibited a concentration dependent potentiation on CCl4-induced lipid peroxidation and on the activity of NADPH-cytochrome c-reductase. The results indicated that piperine potentiated CCl4-induced hepatotoxicity by interacting with liver cells and increased the activity of NADPH-cytochrome c reductase. The increase in activity of this enzyme accelerated biotransformation of CCl4, thereby increasing lipid peroxidation and enhancing hepatotoxicity. © 1995 Informa UK Ltd All rights reserved: reproduction in whole or part not permitted.