Publication: A robust model of natural hepatitis C infection using hepatocyte-like cells derived from human induced pluripotent stem cells as a long-term host
Issued Date
2016
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eng
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Mahidol University
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BioMed Central
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Virology Journal. Vol. 13, (2016), 59
Suggested Citation
Khanit Sa-ngiamsuntorn, Adisak Wongkajornsilp, Phetcharat Phanthong, Suparerk Borwornpinyo, Narisorn Kitiyanant, Wasun Chantratita, Suradej Hongeng A robust model of natural hepatitis C infection using hepatocyte-like cells derived from human induced pluripotent stem cells as a long-term host. Virology Journal. Vol. 13, (2016), 59. doi:10.1186/s12985-016-0519-1 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/2674
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Title
A robust model of natural hepatitis C infection using hepatocyte-like cells derived from human induced pluripotent stem cells as a long-term host
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Abstract
Background: Hepatitis C virus (HCV) could induce chronic liver diseases and hepatocellular carcinoma in human.
The use of primary human hepatocyte as a viral host is restrained with the scarcity of tissue supply. A culture model
restricted to HCV genotype 2a (JFH-1) has been established using Huh7-derived hepatocyte. Other genotypes including
the wild-type virus could not propagate in Huh7, Huh7.5 and Huh7.5.1 cells.
Methods: Functional hepatocyte-like cells (HLCs) were developed from normal human iPS cells as a host for HCV
infection. Mature HLCs were identified for selective hepatocyte markers, CYP450s, HCV associated receptors and HCV
essential host factors. HLCs were either transfected with JFH-1 HCV RNA or infected with HCV particles derived from
patient serum. The enhancing effect of α-tocopherol and the inhibitory effects of INF-α, ribavirin and sofosbuvir to HCV
infection were studied. The HCV viral load and HCV RNA were assayed for the infection efficiency.
Results: The fully-developed HLCs expressed phase I, II, and III drug-metabolizing enzymes, HCV associated receptors
(claudin-1, occludin, CD81, ApoE, ApoB, LDL-R) and HCV essential host factors (miR-122 and SEC14L2) comparable to
the primary human hepatocyte. SEC14L2, an α-tocopherol transfer protein, was expressed in HLCs, but not in Huh7 cell,
had been implicated in effective HCVser infection. The HLCs permitted not only the replication of HCV RNA, but also
the production of HCV particles (HCVcc) released to the culture media. HLCs drove higher propagation of HCVcc
derived from JFH-1 than did the classical host Huh7 cells. HLCs infected with either JFH-1 or wild-type HCV expressed
HCV core antigen, NS5A, NS5B, NS3 and HCV negative-stand RNA. HLCs allowed entire HCV life cycle derived from
either JFH-1, HCVcc or wild-type HCV (genotype 1a, 1b, 3a, 3b, 6f and 6n). Further increasing the HCVser infection in
HLCs was achieved by incubating cell with α-tocopherol. The supernatant from infected HLCs could infect both naïve
HLC and Huh7 cell. Treating infected HLC with INF-α and ribavirin decreased HCV RNA in both the cellular fraction and
the culture medium. The HLCs reacted to HCVcc or wild-type HCV infection by upregulating TNF-α, IL-28B and IL-29.
Conclusions: This robust cell culture model for serum-derived HCV using HLCs as host cells provides a remarkable
system for investigating HCV life cycle, HCV-associated hepatocellular carcinoma development and the screening for
new anti HCV drugs.