Publication: Reduction of the Carbapenemase Inactivation Method (CIM) assay time by real-time PCR
Issued Date
2020-11-01
Resource Type
ISSN
18728359
01677012
01677012
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2-s2.0-85092451474
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Mahidol University
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SCOPUS
Bibliographic Citation
Journal of Microbiological Methods. Vol.178, (2020)
Suggested Citation
F. Haque, S. Fisseha, P. Athamanolap, R. Tower, J. Ortega, C. Dominguez, T. Maruca, D. Torpey, R. Myers, P. Laksanalamai Reduction of the Carbapenemase Inactivation Method (CIM) assay time by real-time PCR. Journal of Microbiological Methods. Vol.178, (2020). doi:10.1016/j.mimet.2020.106072 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/59857
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Title
Reduction of the Carbapenemase Inactivation Method (CIM) assay time by real-time PCR
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Abstract
© 2020 Elsevier B.V. Carbapenemase Inactivation Method (CIM) is a test to detect presence of the carbapenemase in Gram-negative bacteria. Determination of the carbapenemase production by inactivation of meropenem requires that a zone of control E. coli inhibition be measured approximately 6–24 h after plating. We have modified the CIM test by developing a rapid method which instead measures the growth of E. coli indicator strain ATCC 25922 using real-time PCR, referred to as a nucleic acid testing CIM (natCIM). Our natCIM, therefore reduces the detecting time from 6 to 24 h to approximately 4 h.