Use of in vivo infected spleen cells to study Fv-2^r-mediated resistance of mice to friend spleen focus-forming virus

Abstract

In vivo infection of DBA/2 and D2.Fv-2rmice with 105FFU of FV resulted in a rapid adsorption of virus onto the spleen cells. Thus, these cells could be used in the analysis of Fv-2 expression on FV target cells without any difficulty in avoiding extracellular FV particles. Higher frequencies of cells capable of forming IC could be detected in the spleen than in the bone marrow at 2 and 48 h post infection and levels were very low in D2.Fv-2rmice. When recently infected cells were transferred to secondary Fv-2srecipients shortly after infection had been established, the virus-replicating cells released SFFV equally well regardless of whether they originated from Fv-2sor Fv-2rmice. This suggests that suppression of SFFV replication may not take place in Fv-2rcells at an early stage of infection. © 1982.