Publication: Optimization of cyclodextrin production from sago starch
Issued Date
2004-01-01
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ISSN
09608524
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2-s2.0-0344393491
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Mahidol University
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SCOPUS
Bibliographic Citation
Bioresource Technology. Vol.92, No.1 (2004), 49-54
Suggested Citation
Nisanart Charoenlap, Saovanee Dharmsthiti, Sarote Sirisansaneeyakul, Sittiwat Lertsiri Optimization of cyclodextrin production from sago starch. Bioresource Technology. Vol.92, No.1 (2004), 49-54. doi:10.1016/j.biortech.2003.07.007 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/21260
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Title
Optimization of cyclodextrin production from sago starch
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Abstract
Cyclodextrin (CD) is synthesized by bacterial cyclodextrin glycosyltransferase (CGTase) and is widely used in food, pharmaceutical, cosmetic, and agricultural industries. In this study, Bacillus circulans CGTase was partially purified by ammonium sulfate precipitation at 50-70% saturation. The optimum pH and temperature for CD production from sago starch were found to be in the ranges of 4.5-5.0 and 55-60°C, respectively. β-CD was the predominant product, constituting 65% of all CD products. The β-CD produced using partially purified and crude CGTase were compared and found to have no significant difference in yield and productivity. The appropriate proportion of CGTase to sago starch for β-CD production was determined by response surface methodology. The most appropriate enzyme:substrate ratio was 50 Ug sago starch-1CGTase and 60 gl-1sago starch. © 2003 Elsevier Ltd. All rights reserved.