Field Evaluation of a Novel Colorimetric Method - Double-Site Enzyme-Linked Lactate Dehydrogenase Immunodetection Assay - To Determine Drug Susceptibilities of Plasmodium falciparum Clinical Isolates from Northwestern Thailand
Antimicrobial Agents and Chemotherapy. Vol.48, No.4 (2004), 1426-1429
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Alan Brockman, Sittaporn Singlam, Lucy Phiaphun, Sornchai Looareesuwan, Nicholas J. White, François Nosten Field Evaluation of a Novel Colorimetric Method - Double-Site Enzyme-Linked Lactate Dehydrogenase Immunodetection Assay - To Determine Drug Susceptibilities of Plasmodium falciparum Clinical Isolates from Northwestern Thailand. Antimicrobial Agents and Chemotherapy. Vol.48, No.4 (2004), 1426-1429. doi:10.1128/AAC.48.4.1426-1429.2004 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/21686
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Field Evaluation of a Novel Colorimetric Method - Double-Site Enzyme-Linked Lactate Dehydrogenase Immunodetection Assay - To Determine Drug Susceptibilities of Plasmodium falciparum Clinical Isolates from Northwestern Thailand
A double-site enzyme-linked lactate dehydrogenase enzyme immunodetection assay was tested against field isolates of Plasmodium falciparum for assessing in vitro drug susceptibilities to a wide range of antimalarial drugs. Its sensitivity allowed the use of parasite densities as low as 200 parasites/μl of blood. Being a nonisotopic, colorimetric assay, it lies within the capabilities of a modest laboratory at the district level.
