Publication:
Field Evaluation of a Novel Colorimetric Method - Double-Site Enzyme-Linked Lactate Dehydrogenase Immunodetection Assay - To Determine Drug Susceptibilities of Plasmodium falciparum Clinical Isolates from Northwestern Thailand

1

Issued Date

2004-04-01

Resource Type

Article

ISSN

00664804

DOI

10.1128/AAC.48.4.1426-1429.2004

Other identifier(s)

2-s2.0-1642543069

Rights

Mahidol University

Rights Holder(s)

SCOPUS

Bibliographic Citation

Antimicrobial Agents and Chemotherapy. Vol.48, No.4 (2004), 1426-1429

Suggested Citation

Alan Brockman, Sittaporn Singlam, Lucy Phiaphun, Sornchai Looareesuwan, Nicholas J. White, François Nosten Field Evaluation of a Novel Colorimetric Method - Double-Site Enzyme-Linked Lactate Dehydrogenase Immunodetection Assay - To Determine Drug Susceptibilities of Plasmodium falciparum Clinical Isolates from Northwestern Thailand. Antimicrobial Agents and Chemotherapy. Vol.48, No.4 (2004), 1426-1429. doi:10.1128/AAC.48.4.1426-1429.2004 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/21686

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Title

Field Evaluation of a Novel Colorimetric Method - Double-Site Enzyme-Linked Lactate Dehydrogenase Immunodetection Assay - To Determine Drug Susceptibilities of Plasmodium falciparum Clinical Isolates from Northwestern Thailand

Author(s)

Alan Brockman
 Sittaporn Singlam
 Lucy Phiaphun
 Sornchai Looareesuwan
 Nicholas J. White
 François Nosten

Other Contributor(s)

Shoklo Malaria Research Unit
 Mahidol University
 University of Queensland
 John Radcliffe Hospital
 Menzies School of Health Research

Abstract

A double-site enzyme-linked lactate dehydrogenase enzyme immunodetection assay was tested against field isolates of Plasmodium falciparum for assessing in vitro drug susceptibilities to a wide range of antimalarial drugs. Its sensitivity allowed the use of parasite densities as low as 200 parasites/μl of blood. Being a nonisotopic, colorimetric assay, it lies within the capabilities of a modest laboratory at the district level.

Keyword(s)

Medicine

Availability

URI

https://repository.li.mahidol.ac.th/handle/123456789/21686

Collections

Scopus 2001-2005