Publication: Rapid electrochemical detection of coronavirus SARS-CoV-2
24
Issued Date
2021-12-01
Resource Type
ISSN
20411723
Other identifier(s)
2-s2.0-85100539026
Rights
Mahidol University
Rights Holder(s)
SCOPUS
Bibliographic Citation
Nature Communications. Vol.12, No.1 (2021)
Suggested Citation
Thanyarat Chaibun, Jiratchaya Puenpa, Tatchanun Ngamdee, Nimaradee Boonapatcharoen, Pornpat Athamanolap, Anthony Peter O’Mullane, Sompong Vongpunsawad, Yong Poovorawan, Su Yin Lee, Benchaporn Lertanantawong Rapid electrochemical detection of coronavirus SARS-CoV-2. Nature Communications. Vol.12, No.1 (2021). doi:10.1038/s41467-021-21121-7 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/75953
Research Projects
Organizational Units
Authors
Journal Issue
Thesis
Title
Rapid electrochemical detection of coronavirus SARS-CoV-2
Abstract
Coronavirus disease 2019 (COVID-19) is a highly contagious disease caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Diagnosis of COVID-19 depends on quantitative reverse transcription PCR (qRT-PCR), which is time-consuming and requires expensive instrumentation. Here, we report an ultrasensitive electrochemical biosensor based on isothermal rolling circle amplification (RCA) for rapid detection of SARS-CoV-2. The assay involves the hybridization of the RCA amplicons with probes that were functionalized with redox active labels that are detectable by an electrochemical biosensor. The one-step sandwich hybridization assay could detect as low as 1 copy/μL of N and S genes, in less than 2 h. Sensor evaluation with 106 clinical samples, including 41 SARS-CoV-2 positive and 9 samples positive for other respiratory viruses, gave a 100% concordance result with qRT-PCR, with complete correlation between the biosensor current signals and quantitation cycle (Cq) values. In summary, this biosensor could be used as an on-site, real-time diagnostic test for COVID-19.