Publication:
Effects of ATP, cAMP and pH on the initiation of flagellar movement in demembranated models of rat epididymal spermatozoa

Issued Date

1982-01-01

Resource Type

Article

ISSN

00144827

DOI

10.1016/0014-4827(82)90397-4

Other identifier(s)

2-s2.0-0020448799

Rights

Mahidol University

Rights Holder(s)

SCOPUS

Bibliographic Citation

Experimental Cell Research. Vol.142, No.2 (1982), 495-499

Suggested Citation

Napa Treetipsatit, Montri Chulavatnatol Effects of ATP, cAMP and pH on the initiation of flagellar movement in demembranated models of rat epididymal spermatozoa. Experimental Cell Research. Vol.142, No.2 (1982), 495-499. doi:10.1016/0014-4827(82)90397-4 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/30296

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Title

Effects of ATP, cAMP and pH on the initiation of flagellar movement in demembranated models of rat epididymal spermatozoa

Author(s)

Napa Treetipsatit
 Montri Chulavatnatol

Other Contributor(s)

Mahidol University

Abstract

Demembranated model of rat epididymal spermatozoa was employed to establish the conditions for the initiation of flagellar movement. Extensive initiation of the flagellar movement required 0.5 mM ATP, 1 μM cAMP and pH 7.9. The requirement for ATP was highly specific and can partially be replaced by 2′-deoxy-ATP only, but not by analogs of ATP or other nucleoside triphosphates. In contrast, the cAMP requirement was less specific and can partially be replaced by other cyclic nucleotides and cAMP-analogs except 2′-deoxy-cAMP and 2′,3′-cAMP. The data implied that the intracellular pH rise, not the cAMP increase, was the probable trigger for the initiation of sperm motility after ejaculation. During sperm maturation, the sperm motile apparatus appeared unchanged with respect to the above conditions of reactivation. © 1982.

Keyword(s)

Biochemistry, Genetics and Molecular Biology

Availability

URI

https://repository.li.mahidol.ac.th/handle/123456789/30296

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Scopus 1969-1990