Publication: Induced pluripotent stem cell line MUSIi006-A derived from hair follicle keratinocytes as a non-invasive somatic cell source
Issued Date
2018-08-01
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ISSN
18767753
18735061
18735061
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2-s2.0-85050001250
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Mahidol University
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SCOPUS
Bibliographic Citation
Stem Cell Research. Vol.31, (2018), 79-82
Suggested Citation
Bootsakorn Boonkaew, Lunnathaya Tapeng, Ratchapong Netsrithong, Chinnavuth Vatanashevanopakorn, Kovit Pattanapanyasat, Methichit Wattanapanitch Induced pluripotent stem cell line MUSIi006-A derived from hair follicle keratinocytes as a non-invasive somatic cell source. Stem Cell Research. Vol.31, (2018), 79-82. doi:10.1016/j.scr.2018.07.007 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/45095
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Title
Induced pluripotent stem cell line MUSIi006-A derived from hair follicle keratinocytes as a non-invasive somatic cell source
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Abstract
© 2018 The Authors In this study, we used hair follicle keratinocytes for reprogramming. Collection of plucked hairs offers advantages over other somatic cells because no medical professional or operation room is required. Keratinocytes were isolated from plucked hairs of a 21-year-old healthy woman and characterized for the expression of cytokeratin 14 (CK14). Reprogramming of keratinocytes was performed using Sendai virus. Further characterization of the keratinocyte-derived iPSC line (designated as MUSIi006-A) confirmed that the cell line was pluripotent, free from Sendai viral genome and transgenes, and retained normal karyotype. Our method represents an easy, non-invasive and efficient approach for iPSC generation from hair samples.