Publication: Export of a Vibrio parahaemolyticus toxin by the Sec and type III secretion machineries in tandem
Issued Date
2019-05-01
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ISSN
20585276
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2-s2.0-85061729397
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Mahidol University
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SCOPUS
Bibliographic Citation
Nature Microbiology. Vol.4, No.5 (2019), 781-788
Suggested Citation
Shigeaki Matsuda, Ryu Okada, Sarunporn Tandhavanant, Hirotaka Hiyoshi, Kazuyoshi Gotoh, Tetsuya Iida, Toshio Kodama Export of a Vibrio parahaemolyticus toxin by the Sec and type III secretion machineries in tandem. Nature Microbiology. Vol.4, No.5 (2019), 781-788. doi:10.1038/s41564-019-0368-y Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/50191
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Title
Export of a Vibrio parahaemolyticus toxin by the Sec and type III secretion machineries in tandem
Abstract
© 2019, The Author(s), under exclusive licence to Springer Nature Limited. Many Gram-negative pathogens utilize dedicated secretion systems to export virulence factors such as exotoxins and effectors 1–4 . Several exotoxins are synthesized as precursors containing amino-terminal Sec signal peptides and are exported through the inner-membrane-bound Sec machinery to the periplasm, followed by secretion across the outer membrane to the exterior using a type II secretion system (T2SS) 3,5 . Here, we report that thermostable direct haemolysin (TDH), an exotoxin of the food-borne pathogen Vibrio parahaemolyticus, can be exported through the type III secretion system (T3SS), which engages in one-step secretion of effectors 4 , despite possessing a Sec signal peptide and being mainly secreted via the T2SS. Although the precursor of TDH is targeted to the Sec pathway, a fraction of mature TDH was observed to re-enter the bacterial cytoplasm. The N terminus of mature TDH comprises a T3SS signal sequence, allowing it to be loaded into the T3SS. We also show that T3SS-delivered TDH as an effector contributes to intestinal fluid accumulation in a rabbit diarrhoeal model of V. parahaemolyticus infection. Thus, our results show that an unconventional export mechanism for a bacterial toxin via the T3SS in tandem with the Sec machinery facilitates the virulence trait of V. parahaemolyticus.