Publication: Long-term humoral and cellular immune responses elicited by a heterologous Plasmodium vivax apical membrane antigen 1 protein prime/adenovirus boost immunization protocol
Issued Date
2011-09-01
Resource Type
ISSN
10985522
00199567
00199567
Other identifier(s)
2-s2.0-80052309163
Rights
Mahidol University
Rights Holder(s)
SCOPUS
Bibliographic Citation
Infection and Immunity. Vol.79, No.9 (2011), 3642-3652
Suggested Citation
Leoneide Érica Maduro Bouillet, Mariana Oliveira Dias, Natália Alves Dorigo, Andrew Douglas Moura, Bruce Russell, Francois Nosten, Laurent Renia, Érika Martins Braga, Ricardo Tostes Gazzinelli, Maurício M. Rodrigues, Irene S. Soares, Oscar Bruna-Romero Long-term humoral and cellular immune responses elicited by a heterologous Plasmodium vivax apical membrane antigen 1 protein prime/adenovirus boost immunization protocol. Infection and Immunity. Vol.79, No.9 (2011), 3642-3652. doi:10.1128/IAI.05048-11 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/12006
Research Projects
Organizational Units
Authors
Journal Issue
Thesis
Title
Long-term humoral and cellular immune responses elicited by a heterologous Plasmodium vivax apical membrane antigen 1 protein prime/adenovirus boost immunization protocol
Abstract
Apical membrane antigen 1 (AMA-1) is an invasion-related Plasmodium antigen that is expressed during both intracellular and extracellular asexual stages of the parasite's life cycle, making it an ideal target for induction of humoral and cellular immune responses that can protect against malaria. We show here that when it is administered as a recombinant protein (P) in Montanide ISA720 adjuvant, followed by a recombinant human type 5 adenovirus (Ad), intense and long-lasting Plasmodium vivax AMA-1-specific antibody responses (including both IgG1 and IgG2a), as well as proliferative memory T cell responses, can be detected in immunized mice. Memory T cells displayed both central (CD44 hi CD62L hi ) and effector (CD44 hi CD62L lo ) phenotypes, with the central memory phenotype prevailing (56% of AMA-1-specific proliferating cells). Considering the main traits of the memory immune responses induced against AMA-1, this particular sequence of immunogens (P followed by Ad), but no others (Ad/Ad, Ad/P, or P/P), displayed an optimal synergistic effect. These results give further support to the need for preclinical studies of P. vivax vaccine candidate AMA-1 administered in prime/boost protocols that include recombinant proteins and adenoviral vectors. © 2011, American Society for Microbiology.