Publication: Synthesis and Immunological Studies of the Lipomannan Backbone Glycans Found on the Surface of Mycobacterium tuberculosis
Issued Date
2017-07-21
Resource Type
ISSN
15206904
00223263
00223263
Other identifier(s)
2-s2.0-85025170933
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Mahidol University
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SCOPUS
Bibliographic Citation
Journal of Organic Chemistry. Vol.82, No.14 (2017), 7190-7199
Suggested Citation
Harin Leelayuwapan, Niwat Kangwanrangsan, Runglawan Chawengkirttikul, Marisa Ponpuak, Ratthaphol Charlermroj, Kanokthip Boonyarattanakalin, Somsak Ruchirawat, Siwarutt Boonyarattanakalin Synthesis and Immunological Studies of the Lipomannan Backbone Glycans Found on the Surface of Mycobacterium tuberculosis. Journal of Organic Chemistry. Vol.82, No.14 (2017), 7190-7199. doi:10.1021/acs.joc.7b00703 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/42210
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Title
Synthesis and Immunological Studies of the Lipomannan Backbone Glycans Found on the Surface of Mycobacterium tuberculosis
Abstract
© 2017 American Chemical Society. Investigations into novel bacterial drug targets and vaccines are necessary to overcome tuberculosis. Lipomannan (LM), found on the surface of Mycobacterium tuberculosis (Mtb), is actively involved in the pathogenesis and survival of Mtb. Here, we report for the first time a rapid synthesis and biological activities of an LM glycan backbone, α(1-6)mannans. The rapid synthesis is achieved via a regio- and stereoselective ring opening polymerization to generate multiple glycosidic bonds in one simple chemical step, allowing us to finish assembling the defined polysaccharides of 5-20 units within days rather than years. Within the same pot, the polymerization is terminated by a thiol-linker to serve as a conjugation point to carrier proteins and surfaces for immunological experiments. The synthetic glycans are found to have adjuvant activities in vivo. The interactions with DC-SIGN demonstrated the significance of α(1-6)mannan motif present in LM structure. Moreover, surface plasmon resonance (SPR) showed that longer chain of synthetic α(1-6)mannans gain better lectin's binding affinity. The chemically defined components of the bacterial envelope serve as important tools to reveal the interactions of Mtb with mammalian hosts and facilitate the determination of the immunologically active molecular components.