Publication:
Polyacrylamide gel electrophoresis and silver staining for detection of rotavirus in stools from diarrheic patients in Thailand

1

Issued Date

1988-01-01

Resource Type

Article

ISSN

00951137

Other identifier(s)

2-s2.0-0023840539

Rights

Mahidol University

Rights Holder(s)

SCOPUS

Bibliographic Citation

Journal of Clinical Microbiology. Vol.26, No.1 (1988), 158-160

Suggested Citation

S. Kasempimolporn, S. Louisirirotchanakul, P. Sinarachatanant, C. Wasi Polyacrylamide gel electrophoresis and silver staining for detection of rotavirus in stools from diarrheic patients in Thailand. Journal of Clinical Microbiology. Vol.26, No.1 (1988), 158-160. Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/15560

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Title

Polyacrylamide gel electrophoresis and silver staining for detection of rotavirus in stools from diarrheic patients in Thailand

Author(s)

S. Kasempimolporn
 S. Louisirirotchanakul
 P. Sinarachatanant
 C. Wasi

Other Contributor(s)

Mahidol University

Abstract

Detection of rotavirus by polyacrylamide gel electrophoresis in combination with silver staining and by enzyme-linked immunosorbent assay showed 96.7% identical results in tests with 1,304 stool specimens from diarrheic patients. The polyacrylamide gel electrophoresis method can be modified to reduce cost and working time. Phenol extraction of stools, however, is essential in maintaining the sensitivity of the method.

Keyword(s)

Immunology and Microbiology
 Medicine

Availability

URI

https://repository.li.mahidol.ac.th/handle/123456789/15560

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Scopus 1969-1990