Publication: Purification of Gnathostoma spinigerum specific antigen and immunodiagnosis of human gnathostomiasis
Issued Date
1991-01-01
Resource Type
ISSN
00207519
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2-s2.0-0026094419
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Mahidol University
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SCOPUS
Bibliographic Citation
International Journal for Parasitology. Vol.21, No.6 (1991), 677-687
Suggested Citation
Chamnong Nopparatana, Prasert Setasuban, Wanpen Chaicumpa, Pramuan Tapchaisri Purification of Gnathostoma spinigerum specific antigen and immunodiagnosis of human gnathostomiasis. International Journal for Parasitology. Vol.21, No.6 (1991), 677-687. doi:10.1016/0020-7519(91)90079-M Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/22052
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Title
Purification of Gnathostoma spinigerum specific antigen and immunodiagnosis of human gnathostomiasis
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Abstract
Specific antigen of G. spinigerum which has been shown to be a protein with a relative mol. wt of 24,000 (24k) was prepared from the advanced third-stage larvae (L3) obtained from the livers of naturally infected eels. The L3 were ground and extracted with water. Purification procedures involved gel filtration, chromatofocussing and anion exchange column chromatographies, while characterization of the specific antigen was performed by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) and staining, Western blot analysis and isoelectric focussing. The specific antigen which has a pI of 8.5 was used as antigen in the indirect enzyme-linked immunosorbent assay (ELISA) to detect specific antibody in four groups of individuals, namely five parasitologically diagnosed gnathostomiasis patients (group 1); 15 clinically diagnosed gnathostomiasis patients (group 2); 136 patients with other parasitic infections (group 3); and 25 normal healthy parasite-free controls. Sensitivity, specificity and predictive values (positive and negative) of the assay were 100%. © 1991.