Publication: Characterization of TRH/GnRH-like peptides in the sea cucumber, Holothuria scabra, and their effects on oocyte maturation
Issued Date
2020-03-15
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ISSN
00448486
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2-s2.0-85076169061
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Mahidol University
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SCOPUS
Bibliographic Citation
Aquaculture. Vol.518, (2020)
Suggested Citation
Arada Chaiyamoon, Ruchanok Tinikul, Nittiya Nontunha, Supakant Chaichotranunt, Tanes Poomtong, Prasert Sobhon, Yotsawan Tinikul Characterization of TRH/GnRH-like peptides in the sea cucumber, Holothuria scabra, and their effects on oocyte maturation. Aquaculture. Vol.518, (2020). doi:10.1016/j.aquaculture.2019.734814 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/49504
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Title
Characterization of TRH/GnRH-like peptides in the sea cucumber, Holothuria scabra, and their effects on oocyte maturation
Abstract
© 2019 Elsevier B.V. Thyrotropin/Gonadotropin-releasing hormone (TRH/GnRH) is a neurohormone that plays important roles in regulation of several physiological processes of invertebrate species, including echinoderms. The aims of this study were to characterize for the first time a TRH/GnRH-like peptide in the sea cucumber, Holothuria scabra. We have isolated a full-length cDNA for a TRH/GnRH preprohormone gene from the radial nerve cord of H. scabra. We found that H. scabra (hs)TRH/GnRH precursor comprised two types of peptides, namely, the short TRH/GnRH-like peptides and the long TRH/GnRH-like peptides. The expression of hsTRH/GnRH gene, was intense in radial nerve cord, nerve ring and mature ovary by using RT-PCR. By in situ hybridization the transcripts of hsTRH/GnRH was detected at a high level within the late stages (oocyte stages 3 and 4). In the functional assays, injections of the short TRH/GnRH-like peptides could significantly accelerate gonadal development as reflected by increased gonadal-somatic index (GSI), and stimulated gametogenesis, as reflected by significantly increased oocyte proliferation. Taken together, our new discovery of the TRH/GnRH-like peptides in H. scabra contributes important new insights into the evolution of TRH/GnRH neuropeptide system in this sea cucumber species, and these neuropeptides could be potentially applied in the sea cucumber aquaculture.