Publication: C-terminal hydrophobic region leads PRSV P3 protein to endoplasmic reticulum
Issued Date
2007-12-01
Resource Type
ISSN
1572994X
09208569
09208569
Other identifier(s)
2-s2.0-35549004578
Rights
Mahidol University
Rights Holder(s)
SCOPUS
Bibliographic Citation
Virus Genes. Vol.35, No.3 (2007), 611-617
Suggested Citation
Sarasate Eiamtanasate, Mila Juricek, Yun Kiam Yap C-terminal hydrophobic region leads PRSV P3 protein to endoplasmic reticulum. Virus Genes. Vol.35, No.3 (2007), 611-617. doi:10.1007/s11262-007-0114-z Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/24072
Research Projects
Organizational Units
Authors
Journal Issue
Thesis
Title
C-terminal hydrophobic region leads PRSV P3 protein to endoplasmic reticulum
Author(s)
Abstract
P3 protein is one of the least characterized potyviral proteins in both functions and sub-cellular localization. In this study, we examined the sub-cellular localization of PRSV P3 and its intermediate, P3-6K1by expressing their GFP fusion proteins in onion epidermal cells. Our results showed that both P3- and P3-6K1GFP fusion proteins were localized at the endoplasmic reticulum. Deletion analysis indicated that C-terminal of P3 protein contained localization signal, and a 19 amino acids hydrophobic domain from this region was able to target the GFP fusion protein to endoplasmic reticulum. C-terminal of P3 proteins has been suggested to be involved in both viability and pathogenicity of the potyvirus. Therefore, our result suggests that localization of P3 protein at endoplasmic reticulum is essential for functionality of P3 protein. © 2007 Springer Science+Business Media, LLC.
