Publication: In vivo identification of Bacillus thuringiensis Cry4Ba toxin receptors by RNA interference knockdown of glycosylphosphatidylinositol-linked aminopeptidase N transcripts in Aedes aegypti larvae
Issued Date
2011-04-22
Resource Type
ISSN
10902104
0006291X
0006291X
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2-s2.0-79955016662
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Mahidol University
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SCOPUS
Bibliographic Citation
Biochemical and Biophysical Research Communications. Vol.407, No.4 (2011), 708-713
Suggested Citation
Suchada Saengwiman, Aratee Aroonkesorn, Plaipol Dedvisitsakul, Somsri Sakdee, Somphob Leetachewa, Chanan Angsuthanasombat, Kusol Pootanakit In vivo identification of Bacillus thuringiensis Cry4Ba toxin receptors by RNA interference knockdown of glycosylphosphatidylinositol-linked aminopeptidase N transcripts in Aedes aegypti larvae. Biochemical and Biophysical Research Communications. Vol.407, No.4 (2011), 708-713. doi:10.1016/j.bbrc.2011.03.085 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/11564
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Title
In vivo identification of Bacillus thuringiensis Cry4Ba toxin receptors by RNA interference knockdown of glycosylphosphatidylinositol-linked aminopeptidase N transcripts in Aedes aegypti larvae
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Abstract
Bacillus thuringiensis Cry4Ba toxin selectively kills Aedes aegypti mosquito larvae as it is in part due to the presence of specific membrane-bound protein receptors. In this study, using data mining approach, we initially identified three potential glycosylphosphatidylinositol-linked aminopeptidase N (GPI-APN) isoforms, APN2778, APN2783 and APN5808, which are believed to act as Cry4Ba toxin receptors. These three isoforms that are functionally expressed in the larval midgut can be sequence-specific knocked down (ranging from ~80% to 95%) by soaking the Aedes aegypti larvae in buffer of long double-stranded GPI-APN RNAs (~300-680. bp). Finally, to see the physiological effect of APN knockdowns, the larvae were fed with Escherichia coli expressing Cry4Ba toxin. The results revealed that all the three identified GPI-APN isoforms may possibly function as a Cry4Ba receptor, particularly for APN2783 as those larvae with this transcript knockdown showed a dramatic increase in resistance to Cry4Ba toxicity. © 2011 Elsevier Inc.