Publication: Suppression of α-glucosidase gene by RNA interference can increase resistance of Culex quinquefasciatus to Bacillus sphaericus binary toxin
Issued Date
2015-12-02
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ISSN
13600478
09583157
09583157
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2-s2.0-84944059456
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Mahidol University
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SCOPUS
Bibliographic Citation
Biocontrol Science and Technology. Vol.25, No.12 (2015), 1453-1466
Suggested Citation
Warunya Woradulayapinij, Mongkon Audtho, Chanpen Wiwat Suppression of α-glucosidase gene by RNA interference can increase resistance of Culex quinquefasciatus to Bacillus sphaericus binary toxin. Biocontrol Science and Technology. Vol.25, No.12 (2015), 1453-1466. doi:10.1080/09583157.2015.1066310 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/35054
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Title
Suppression of α-glucosidase gene by RNA interference can increase resistance of Culex quinquefasciatus to Bacillus sphaericus binary toxin
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Abstract
© 2015 Taylor & Francis. Bacillus sphaericus binary toxin displays a specific toxicity towards Culex spp. larvae. Resistance of Culex quinquefasciatus to B. sphaericus is associated with the reduction or absence of 66-kDa α-glucosidase, a binary toxin receptor, in larval midgut microvilli. In this study, long double-stranded RNA (dsRNA) targeting the α-glucosidase gene was used to suppress the expression of α-glucosidase in Cx. quinquefasciatus larvae and the consequences of the suppression was investigated. Three sets of dsRNAs, dsGRi1, dsGRi2, and dsGRi3, which are homologous to distinct regions of α-glucosidase gene, were delivered to mosquito larva by feeding with dsRNA–chitosan particles. Reverse transcriptase-polymerase chain reaction results demonstrated that the expression of α-glucosidase mRNA was reduced by 43% in larvae fed with dsGRi3–chitosan, compared to those fed with dsgfp–chitosan particles. Western blot analysis revealed a 7–17% reduction of α-glucosidase protein expression in larvae fed with dsGRi–chitosan. Mortality rates of larvae that received dsRNAs–chitosan particles were only 45–62% compared to those that received dsgfp–chitosan particles. These results indicate that α-glucosidase is essential for the toxicity of B. sphaericus binary toxin to Cx. quinquefasciatus.