Publication: Functional Genetic Analysis Reveals a 2-Alkyl-4-Quinolone Signaling System in the Human Pathogen Burkholderia pseudomallei and Related Bacteria
Issued Date
2006-07-01
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ISSN
10745521
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2-s2.0-33746257392
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Mahidol University
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SCOPUS
Bibliographic Citation
Chemistry and Biology. Vol.13, No.7 (2006), 701-710
Suggested Citation
Stephen P. Diggle, Putthapoom Lumjiaktase, Francesca Dipilato, Klaus Winzer, Mongkol Kunakorn, David A. Barrett, Siri Ram Chhabra, Miguel Cámara, Paul Williams Functional Genetic Analysis Reveals a 2-Alkyl-4-Quinolone Signaling System in the Human Pathogen Burkholderia pseudomallei and Related Bacteria. Chemistry and Biology. Vol.13, No.7 (2006), 701-710. doi:10.1016/j.chembiol.2006.05.006 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/23016
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Title
Functional Genetic Analysis Reveals a 2-Alkyl-4-Quinolone Signaling System in the Human Pathogen Burkholderia pseudomallei and Related Bacteria
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Abstract
Pseudomonas aeruginosa synthesizes diverse 2-alkyl-4(1H)-quinolones (AHQs), including the signaling molecule 2-heptyl-3-hydroxy-4(1H)-quinolone (PQS), via the pqsABCDE locus. By examining the genome databases, homologs of the pqs genes were identified in other bacteria. However, apart from P. aeruginosa, only Burkholderia pseudomallei and B. thailandensis contained a complete pqsA-E operon (termed hhqA-E). By introducing the B. pseudomallei hhqA and hhqE genes into P. aeruginosa pqsA and pqsE mutants, we show that they are functionally conserved and restore virulence factor and PQS production. B. pseudomallei, B. thailandensis, B. cenocepacia, and P. putida each produced 2-heptyl-4(1H)-quinolone (HHQ), but not PQS. Mutation of hhqA in B. pseudomallei resulted in the loss of AHQ production, altered colony morphology, and enhanced elastase production, which was reduced to parental levels by exogenous HHQ. These data reveal a role for AHQs in bacterial cell-to-cell communication beyond that seen in P. aeruginosa. © 2006 Elsevier Ltd. All rights reserved.