Publication: Cloning and expression of the gene encoding solanesyl diphosphate synthase from Hevea brasiliensis
Issued Date
2007-04-01
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ISSN
01689452
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2-s2.0-33847284023
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Mahidol University
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SCOPUS
Bibliographic Citation
Plant Science. Vol.172, No.4 (2007), 824-831
Suggested Citation
Atiphon Phatthiya, Seiji Takahashi, Nopphakaew Chareonthiphakorn, Tanetoshi Koyama, Dhirayos Wititsuwannakul, Rapepun Wititsuwannakul Cloning and expression of the gene encoding solanesyl diphosphate synthase from Hevea brasiliensis. Plant Science. Vol.172, No.4 (2007), 824-831. doi:10.1016/j.plantsci.2006.12.015 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/24018
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Title
Cloning and expression of the gene encoding solanesyl diphosphate synthase from Hevea brasiliensis
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Abstract
Plastoquinones play important roles as electron carriers in the light-dependent reactions of photosynthesis and also as a cofactor of phytoene desaturation in the synthesis of carotenoid. A plastoquinone-9 (PQ-9) was identified in Frey-Wyssling organelles of fresh rubber latex from Hevea brasiliensis. This indicates that a Hevea solanesyl diphosphate synthase (HbSDS) must be present for the synthesis of the C45prenyl side chain of the PQ-9 found in the rubber latex. Based on the sequence information of other trans-prenyl diphosphate synthases, the cDNA encoding HbSDS was cloned from a Hevea rubber latex cDNA library. Sequence analysis revealed the presence of an ORF consisting of 1254 bp capable of encoding a 46,095 Da polypeptide. The deduced amino acid sequence of the clone contained all conserved regions found in typical trans-prenyl chain elongating enzymes. This cDNA, expressed in Escherichia coli cells as an insoluble but Trx-His-tagged fusion protein, showed a distinct solanesyl diphosphate synthase activity in the presence of Triton X-100. The expression level of HbSDS mRNA in latex was found to be higher than those in leaves, stems and roots. The expressed HbSDS is suggested to be a specific enzyme in the Frey-Wyssling particles of rubber latex. © 2007 Elsevier Ireland Ltd. All rights reserved.