Publication: Efficiency of modified medium for direct chromosome preparation of the chorionic villi.
Issued Date
1990-02-01
Resource Type
ISSN
01252208
Other identifier(s)
2-s2.0-0025381484
Rights
Mahidol University
Rights Holder(s)
SCOPUS
Bibliographic Citation
Journal of the Medical Association of Thailand. Vol.73 Suppl 1, (1990), 73-76
Suggested Citation
D. Kangwanpong, S. Ajjimakorn, M. Jirapinyo Efficiency of modified medium for direct chromosome preparation of the chorionic villi.. Journal of the Medical Association of Thailand. Vol.73 Suppl 1, (1990), 73-76. Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/16082
Research Projects
Organizational Units
Authors
Journal Issue
Thesis
Title
Efficiency of modified medium for direct chromosome preparation of the chorionic villi.
Author(s)
Other Contributor(s)
Abstract
The direct chromosome preparation from the CV is a reliable method for diagnosis of the gross chromosomal anomalies and could be completed in the shortest possible time. However, direct villi preparation does not always provide sufficient or good quality metaphases. The culture medium plays an important role in achieving a good success rate. We report the efficacy of our modified medium and compared it with Chang's. The modified medium showed slightly higher successful karyotyping (68.4%) than the Chang medium (65.4%). This success rate showed no statistically significant difference. The success rate enhanced up to 92.3 per cent using our modified medium when the CV was of good quality. The higher amount of CV did not lead to a higher success rate. Any amount more than 5 mg of the CV was sufficient for direct chromosome preparation.