Publication: Differential Expression of Tyrosine Hydroxylase Protein and Apoptosis-Related Genes in Differentiated and Undifferentiated SH-SY5Y Neuroblastoma Cells Treated with MPP<sup>+</sup>
Issued Date
2015-01-01
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ISSN
20901860
20901852
20901852
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2-s2.0-84948395308
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Mahidol University
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SCOPUS
Bibliographic Citation
Neurology Research International. Vol.2015, (2015)
Suggested Citation
Kawinthra Khwanraj, Chareerut Phruksaniyom, Suriyat Madlah, Permphan Dharmasaroja Differential Expression of Tyrosine Hydroxylase Protein and Apoptosis-Related Genes in Differentiated and Undifferentiated SH-SY5Y Neuroblastoma Cells Treated with MPP<sup>+</sup>. Neurology Research International. Vol.2015, (2015). doi:10.1155/2015/734703 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/36611
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Title
Differential Expression of Tyrosine Hydroxylase Protein and Apoptosis-Related Genes in Differentiated and Undifferentiated SH-SY5Y Neuroblastoma Cells Treated with MPP<sup>+</sup>
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Abstract
© 2015 Kawinthra Khwanraj et al. The human neuroblastoma SH-SY5Y cell line has been used as a dopaminergic cell model for Parkinson's disease research. Whether undifferentiated or differentiated SH-SY5Y cells are more suitable remains controversial. This study aims to evaluate the expression of apoptosis-related mRNAs activated by MPP+and evaluate the differential expression of tyrosine hydroxylase (TH) in undifferentiated and retinoic acid-(RA-) induced differentiated cells. The western blot results showed a gradual decrease in TH in undifferentiated cells and a gradual increase in TH in differentiated cells from days 4 to 10 after cell plating. Immunostaining revealed a gradual increase in TH along with neuritic outgrowth in differentiated cells on days 4 and 7 of RA treatment. For the study on cell susceptibility to MPP+and the expression of apoptosis-related genes, MTT assay showed a decrease in cell viability to approximately 50% requiring 500 and 1000 M of MPP+for undifferentiated and RA-differentiated cells, respectively. Using real-time RT-PCR, treatment with 500 M MPP+led to significant increases in the Bax/Bcl-2 ratio, p53, and caspase-3 in undifferentiated cells but was without significance in differentiated cells. In conclusion, differentiated cells may be more suitable, and the shorter duration of RA differentiation may make the SH-SY5Y cell model more accessible.
