Publication: Development of an in vitro assay and demonstration of Plasmodium berghei liver- Stage inhibition by TRAP-specific CD8+ T Cells
Issued Date
2015-03-30
Resource Type
ISSN
19326203
Other identifier(s)
2-s2.0-84926334122
Rights
Mahidol University
Rights Holder(s)
SCOPUS
Bibliographic Citation
PLoS ONE. Vol.10, No.3 (2015)
Suggested Citation
Rhea J. Longley, Karolis Bauza, Katie J. Ewer, Adrian V.S. Hill, Alexandra J. Spencer Development of an in vitro assay and demonstration of Plasmodium berghei liver- Stage inhibition by TRAP-specific CD8+ T Cells. PLoS ONE. Vol.10, No.3 (2015). doi:10.1371/journal.pone.0119880 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/35182
Research Projects
Organizational Units
Authors
Journal Issue
Thesis
Title
Development of an in vitro assay and demonstration of Plasmodium berghei liver- Stage inhibition by TRAP-specific CD8+ T Cells
Abstract
© 2015 Longley et al. The development of an efficacious vaccine against the Plasmodium parasite remains a top priority. Previous research has demonstrated the ability of a prime-boost virally vectored sub-unit vaccination regimen, delivering the liver-stage expressed malaria antigen TRAP, to produce high levels of antigen-specific T cells. The liver-stage of malaria is the main target of T cell-mediated immunity, yet a major challenge in assessing new T cell inducing vaccines has been the lack of a suitable pre-clinical assay. We have developed a flow-cytometry based in vitro T cell killing assay using a mouse hepatoma cell line, Hepa1-6, and Plasmodium berghei GFP expressing sporozoites. Using this assay, P. berghei TRAP-specific CD8+T cell enriched splenocytes were shown to inhibit liver-stage parasites in an ef-fector- to-target ratio dependent manner. Further development of this assay using human hepatocytes and P. falciparum would provide a new method to pre-clinically screen vaccine candidates and to elucidate mechanisms of protection in vitro.