Publication: Application of multiple stepwise spinning disk processing for the synthesis of poly(methyl acrylates) coated chitosan-diclofenac sodium nanoparticles for colonic drug delivery
Issued Date
2013-09-27
Resource Type
ISSN
18790720
09280987
09280987
Other identifier(s)
2-s2.0-84884515405
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Mahidol University
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SCOPUS
Bibliographic Citation
European Journal of Pharmaceutical Sciences. Vol.50, No.3-4 (2013), 303-311
Suggested Citation
Kampanart Huanbutta, Pornsak Sriamornsak, Manee Luangtana-Anan, Sontaya Limmatvapirat, Satit Puttipipatkhachorn, Lee Yong Lim, Katsuhide Terada, Jurairat Nunthanid Application of multiple stepwise spinning disk processing for the synthesis of poly(methyl acrylates) coated chitosan-diclofenac sodium nanoparticles for colonic drug delivery. European Journal of Pharmaceutical Sciences. Vol.50, No.3-4 (2013), 303-311. doi:10.1016/j.ejps.2013.07.010 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/32731
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Title
Application of multiple stepwise spinning disk processing for the synthesis of poly(methyl acrylates) coated chitosan-diclofenac sodium nanoparticles for colonic drug delivery
Abstract
The production of pharmaceutical nanoparticles by the spinning disk processing (SDP) technique has advantages in terms of its scalability and its capacity to produce readily tunable nanoparticles of narrow size distribution. In this study, we successfully developed a novel multiple stepwise SDP technique to develop aggregates of uniformly sized poly(methyl acrylates)-coated chitosan-diclofenac sodium nanocores (CS-PMA NPs) for colonic drug delivery. The processing conditions were optimized using the Box-Behnken design. SEM and TEM micrographs showed the optimized system to consist of 10 μm-sized agglomerates of CS-PMA NPs, the latter measuring 10 nm in diameter. High drug entrapment of 88% was attained. Potential colon-targeted drug release from the CS-PMA NPs was demonstrated, with retardation of drug release in simulated gastrointestinal fluids and over 90% of the drug load released into simulated colonic fluid within 8 h. Drug uptake from CS-PMA NPs into Caco-2 cells was threefold higher than that from a control drug solution, with no apparent cytotoxicity observed at the NP doses administered. The collective data suggest that the SDP is a robust manufacturing method that can potentially be used to scale up the production of composite nanoparticulate colon-targeted drug delivery systems. © 2013 Elsevier B.V. All rights reserved.