Publication: Contrasting signatures of selection on the Plasmodium falciparum erythrocyte binding antigen gene family
Issued Date
2006-10-01
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ISSN
01666851
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2-s2.0-33748028816
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Mahidol University
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SCOPUS
Bibliographic Citation
Molecular and Biochemical Parasitology. Vol.149, No.2 (2006), 182-190
Suggested Citation
Federica Verra, Watcharee Chokejindachai, Gareth D. Weedall, Spencer D. Polley, Tabitha W. Mwangi, Kevin Marsh, David J. Conway Contrasting signatures of selection on the Plasmodium falciparum erythrocyte binding antigen gene family. Molecular and Biochemical Parasitology. Vol.149, No.2 (2006), 182-190. doi:10.1016/j.molbiopara.2006.05.010 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/22972
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Title
Contrasting signatures of selection on the Plasmodium falciparum erythrocyte binding antigen gene family
Abstract
Erythrocyte binding antigens of Plasmodium falciparum are involved in erythrocyte invasion, and may be targets of acquired immunity. Of the five eba genes, protein products have been detected for eba-175, eba-181 and eba-140, but not for ψeba-165 or ebl-1, providing opportunity for comparative analysis of genetic variation to identify selection. Region II of each of these genes was sequenced from a cross-sectional sample of parasites in an endemic Kenyan population, and the frequency distributions of polymorphisms analysed. A positive value of Tajima's D was observed for eba-175 (D = 1.13) indicating an excess of intermediate frequency polymorphisms, while all other genes had negative values, the most negative being ebl-1 (D = -2.35) followed by ψeba-165 (D = -1.79). The eba-175 and ebl-1 genes were then studied in a sample of parasites from Thailand, for which a positive Tajima's D value was again observed for eba-175 (D = 1.79), and a negative value for ebl-1 (D = -1.85). This indicates that eba-175 is under balancing selection in each population, in strong contrast to the other members of the gene family, particularly ebl-1 and ψeba-165 that may have been under recent directional selection. Population expansion simulations were performed under a neutral model, further supporting the departures from neutrality of these genes. © 2006 Elsevier B.V. All rights reserved.