Publication: Structure elucidation of glykenin glycosidic antibiotics from Basidiomycetes sp. V. High-performance liquid chromatographic separation of components of glykenin
Issued Date
1994-04-01
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ISSN
00219673
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2-s2.0-0028344959
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Mahidol University
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SCOPUS
Bibliographic Citation
Journal of Chromatography A. Vol.664, No.2 (1994), 195-202
Suggested Citation
Fumiko Nishida, Mika Nishimura, Ken Ichi Harada, Makoto Suzuki, Vithaya Meevootisom, Timothy W. Flegel, Yodhathai Thebtaranonth, Suthum Intararuangsorn Structure elucidation of glykenin glycosidic antibiotics from Basidiomycetes sp. V. High-performance liquid chromatographic separation of components of glykenin. Journal of Chromatography A. Vol.664, No.2 (1994), 195-202. doi:10.1016/0021-9673(94)87008-X Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/9514
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Title
Structure elucidation of glykenin glycosidic antibiotics from Basidiomycetes sp. V. High-performance liquid chromatographic separation of components of glykenin
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Abstract
The glycosidic antibiotics of the glykenin (GK) family produced by Basidiomycetes sp. were separated into nine components (GK-I-VII and DG) by normal-phase chromatography. It was found that these components differ in the number and location of the acetyl groups in the sugar moiety. Each component (GK-I-VII and DG) was further separated into three isomers (A, B and C), which possess different aglycones, by reversed-phase chromatography on an ODS column with methanol-acetonitrile as eluent. The best composition of the eluent was found to be methanol-acetonitrile-1% trifluoroacetic acid (4:3.5:2.5). The profile analysis of GK-III-VII and DG was also carried out using a modified mobile phase. The combination of normal- and reversed-phase chromatography separated all components of the GK mixture except GK-I and II. The relationship between structure and separation behaviour of GK is discussed. © 1994.