Publication: Hepatic mitochondrial function and lysosomal enzyme activity in ethanol-potentiated aflatoxin B1 hepatotoxicity
Issued Date
1990-01-01
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ISSN
03784274
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2-s2.0-0025352977
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Mahidol University
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SCOPUS
Bibliographic Citation
Toxicology Letters. Vol.52, No.2 (1990), 179-190
Suggested Citation
Chaivat Toskulkao, Thirayudh Glinsukon Hepatic mitochondrial function and lysosomal enzyme activity in ethanol-potentiated aflatoxin B1 hepatotoxicity. Toxicology Letters. Vol.52, No.2 (1990), 179-190. doi:10.1016/0378-4274(90)90152-C Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/16170
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Title
Hepatic mitochondrial function and lysosomal enzyme activity in ethanol-potentiated aflatoxin B1 hepatotoxicity
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Abstract
The possible role of hepatic mitochondrial function and lysosomal enzyme activity in ethanol-enhanced aflatoxin B 1 (AFB 1 ) hepatotoxicity was studied in male rats. Hepatic ATP content was significantly decreased in rats treated with ethanol (4.0 g/kg body wt.) and AFB 1 (2.0 mg/kg body wt.) compared with rats treated with AFB 1 alone at 12-72 h after AFB 1 administration. The decrease in hepatic ATP content was due to the decrease in the activity of NADH-cytochrome c reductase whereas cytochrome oxidase activity did not differ in rats treated with ethanol and AFB 1 when compared to AFB 1 alone. Total and free activities of hepatic lysosomal enzymes (glucuronidase, arylsulfatase and acid phosphatase) were significantly increased in rats treated with ethanol and AFB 1 at 24-36 h after AFB 1 administration when compared to AFB 1 alone. The increase in hepatic lysosomal enzyme activities correlated well with the increase in the lipid peroxide level of lysosomes in rats treated with ethanol and AFB 1 . These findings indicate that the decrease in hepatic mitochondrial respiratory enzyme activities and the increase in lipid peroxide level of lysosomes might lead to a decrease in hepatic ATP content, and that the increase in the activities of hepatic lysosomal enzymes, respectively, enhance the AFB 1 hepatotoxicity of ethanol. © 1990.