Publication: Vaccine potential of recombinant cathepsin B against Fasciola gigantica
Issued Date
2013-09-01
Resource Type
ISSN
10902449
00144894
00144894
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2-s2.0-84880422630
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Mahidol University
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SCOPUS
Bibliographic Citation
Experimental Parasitology. Vol.135, No.1 (2013), 102-109
Suggested Citation
Pathanin Chantree, Manussabhorn Phatsara, Krai Meemon, Pannigan Chaichanasak, Narin Changklungmoa, Pornanan Kueakhai, Natcha Lorsuwannarat, Kant Sangpairoj, Sineenart Songkoomkrong, Chaitip Wanichanon, Tadashi Itagaki, Prasert Sobhon Vaccine potential of recombinant cathepsin B against Fasciola gigantica. Experimental Parasitology. Vol.135, No.1 (2013), 102-109. doi:10.1016/j.exppara.2013.06.010 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/31874
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Title
Vaccine potential of recombinant cathepsin B against Fasciola gigantica
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Abstract
In Fasciola gigantica, cathepsin Bs, especially cathepsin B2 and B3 are expressed in early juvenile stages, and are proposed to mediate the invasion of host tissues. Thus they are thought to be the target vaccine candidates that can block the invasion and migration of the juvenile parasite. To evaluate their vaccine potential, the recombinant cathepsin B2 (rFgCatB2) and cathepsin B3 (rFgCatB3) were expressed in yeast, Pichia pastoris, and used to immunize mice in combination with Freund's adjuvant to evaluate the protection against the infection by F. gigantica metacercariae, and the induction of immune responses. Mice immunized with both recombinant proteins exhibited high percent of parasite reduction at 60% for rFgCatB2 and 66% for rFgCatB3. Immunization by both antigens induced continuously increasing levels of IgG1 and IgG2a with a higher level of IgG1 isotype, indicating the mixed Th1/Th2 responses with Th2 predominating. When examined individually, the higher levels of IgG1 and IgG2a were correlated with the lower numbers of worm recoveries. Thus, both cathepsin B2 and cathepsin B3 are plausible vaccine candidates whose potential should be further tested in large economic animals. © 2013 Elsevier Inc.