Publication: Generation of two induced pluripotent stem cell lines (MUSIi011-A and MUSIi011-B) from peripheral blood T lymphocytes of a healthy individual
Issued Date
2019-08-01
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ISSN
18767753
18735061
18735061
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2-s2.0-85067458253
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Mahidol University
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SCOPUS
Bibliographic Citation
Stem Cell Research. Vol.39, (2019)
Suggested Citation
Ratchapong Netsrithong, Nutchanawan Promnakhon, Bootsakorn Boonkaew, Chinnavuth Vatanashevanopakorn, Kovit Pattanapanyasat, Methichit Wattanapanitch Generation of two induced pluripotent stem cell lines (MUSIi011-A and MUSIi011-B) from peripheral blood T lymphocytes of a healthy individual. Stem Cell Research. Vol.39, (2019). doi:10.1016/j.scr.2019.101487 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/50113
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Title
Generation of two induced pluripotent stem cell lines (MUSIi011-A and MUSIi011-B) from peripheral blood T lymphocytes of a healthy individual
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Abstract
© 2019 Activated T lymphocytes of a healthy individual were reprogrammed to induced pluripotent stem cells (iPSCs) using Sendai viral vectors. Two iPSC lines, MUSIi011-A and MUSIi011-B, were established and characterized for the expression of pluripotent markers. Both iPSC lines were able to differentiate into cells of three embryonic germ layers via embryoid body formation, exhibited normal karyotypes and were free of viral genome and transgenes at passage 15. These T lymphocyte-derived iPSCs (T-iPSCs) represent a useful starting cell source for developing next-generation immune cells such as chimeric antigen receptor (CAR)-engineered iPSC-derived T lymphocytes for the application in adoptive immunotherapy.