Publication:
Authentication of Coscinium fenestratum among the other Menispermaceae plants prescribed in Thai folk medicines

dc.contributor.authorRith Watthanachaiyingcharoenen_US
dc.contributor.authorKatsuko Komatsuen_US
dc.contributor.authorShu Zhuen_US
dc.contributor.authorOpa Vajraguptaen_US
dc.contributor.authorWichet Leelamaniten_US
dc.contributor.otherSrinakharinwirot Universityen_US
dc.contributor.otherUniversity of Toyamaen_US
dc.contributor.otherMahidol Universityen_US
dc.date.accessioned2018-09-24T09:43:14Z
dc.date.available2018-09-24T09:43:14Z
dc.date.issued2010-01-01en_US
dc.description.abstractIn Ayurveda and Thai traditional medicines, material from Coscinium fenestratum is commonly prescribed as active ingredients with diverse therapeutic purposes. However, C. fenestratum is also a seriously endangered medicinal liana. Thus, its crude material is very rare and is being substituted with substances from Arcangelisia flava or Fibraurea tinctoria (Menispermaceae), which have high morphological similarity. In this current study, nuclear 18S ribosomal RNA (rRNA) gene and nuclear ribosomal DNA internal transcribed spacer (ITS) gene sequences with the polymerase chain reaction-restriction fragment length polymorphisms (PCR-RFLPs) technique were exploited to identify these three species. The nuclear 18S rRNA gene sequences of C. fenestratum, A. flava, and F. tinctoria consisted of 1809, 1805, and 1809 base pairs (bps), respectively, while their ITS gene regions were 694, 622, and 631 bps in length, respectively. The 18S rRNA gene of C. fenestratum digested with SmaI restriction enzyme displayed the electrophoresis profile of 729 and 790 bps; for A. flava and F. tinctoria, the digested products showed fragments of 1519 bps. Although the ITS gene regions of A. flava and F. tinctoria had unrecognized sequences with SalI, the SalI-digested ITS of C. fenestratum exhibited fragments of approximately 599 bp. Thus, the 18S rRNA gene and ITS gene sequences with PCR-RFLPs were proven to be powerful molecular markers for identifying C. fenestratum and distinguishing it from the other two Menispermaceae plants. © 2010 Pharmaceutical Society of Japan.en_US
dc.identifier.citationBiological and Pharmaceutical Bulletin. Vol.33, No.1 (2010), 91-94en_US
dc.identifier.doi10.1248/bpb.33.91en_US
dc.identifier.issn13475215en_US
dc.identifier.issn09186158en_US
dc.identifier.other2-s2.0-75149167072en_US
dc.identifier.urihttps://repository.li.mahidol.ac.th/handle/20.500.14594/29933
dc.rightsMahidol Universityen_US
dc.rights.holderSCOPUSen_US
dc.source.urihttps://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=75149167072&origin=inwarden_US
dc.subjectPharmacology, Toxicology and Pharmaceuticsen_US
dc.titleAuthentication of Coscinium fenestratum among the other Menispermaceae plants prescribed in Thai folk medicinesen_US
dc.typeArticleen_US
dspace.entity.typePublication
mu.datasource.scopushttps://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=75149167072&origin=inwarden_US

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