Publication: Magnetic nanoparticle formulations for DNA and siRNA delivery
Issued Date
2007-04-01
Resource Type
ISSN
03048853
Other identifier(s)
2-s2.0-33947115312
Rights
Mahidol University
Rights Holder(s)
SCOPUS
Bibliographic Citation
Journal of Magnetism and Magnetic Materials. Vol.311, No.1 SPEC. ISS. (2007), 275-281
Suggested Citation
Olga Mykhaylyk, Dialekti Vlaskou, Nittaya Tresilwised, Pimolpan Pithayanukul, Winfrid Möller, Christian Plank Magnetic nanoparticle formulations for DNA and siRNA delivery. Journal of Magnetism and Magnetic Materials. Vol.311, No.1 SPEC. ISS. (2007), 275-281. doi:10.1016/j.jmmm.2006.10.1178 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/24607
Research Projects
Organizational Units
Authors
Journal Issue
Thesis
Title
Magnetic nanoparticle formulations for DNA and siRNA delivery
Abstract
Newly synthesized magnetic nanomaterials possess high DNA binding capacity either itself or in the presence of a positively charged lipid-based Metafectene™ reagent or branched polyethylene imine 25 kDa. Polyethylene imine (PEI)-modified nanomaterials are able to deliver nucleic acids in cell culture in duplexes. Magnetofection with triplexes of nanomaterials results in higher transduction efficiencies compared to optimal PEI or Metafectene formulations. 90% transient down-regulation of the target protein in HeLa-green fluorescence protein cells was achieved at short interfering RNA concentrations as low as 8 nM with a formulation of PEI-modified nanoparticles. © 2006 Elsevier B.V. All rights reserved.