Publication: In-vitro cultivation: A sensitive method for detecting Blastocystis hominis
Issued Date
2002-12-01
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ISSN
00034983
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2-s2.0-0036976388
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Mahidol University
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SCOPUS
Bibliographic Citation
Annals of Tropical Medicine and Parasitology. Vol.96, No.8 (2002), 803-807
Suggested Citation
S. Leelayoova, P. Taamasri, R. Rangsin, T. Naaglor, U. Thathaisong, M. Mungthin In-vitro cultivation: A sensitive method for detecting Blastocystis hominis. Annals of Tropical Medicine and Parasitology. Vol.96, No.8 (2002), 803-807. doi:10.1179/000349802125002275 Retrieved from: https://repository.li.mahidol.ac.th/handle/20.500.14594/20181
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Title
In-vitro cultivation: A sensitive method for detecting Blastocystis hominis
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Abstract
Currently, the detection of human infection with Blastocystis hominis is usually based on the examination under a light microscope of faecal samples, either directly, as 'simple smears', or after some form of concentration. Whether short-term, in-vitro cultivation would increase the sensitivity of such detection remains a matter of controversy. Over 900 fresh stool specimens, from soldiers in the Royal Thai Army, were each checked for the parasite using three methods: simple smears; formalin-ethyl-acetate concentration; and cultivation in Jones' medium. Although 334 of the samples were found to be culture-positive, the parasites were only detected in 142 of the simple smears, and faecal concentration led to an even lower sensitivity (64 positive samples). In-vitro cultivation does seem worthwhile in the detection of B. hominis carriage in field studies.