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A piezoelectric-based immunosensor for high density lipoprotein particle measurement

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dc.contributor.authorSuticha Chuntaen_US
dc.contributor.authorJamikorn Suk-Anakeen_US
dc.contributor.authorKosum Chansirien_US
dc.contributor.authorChamras Promptmasen_US
dc.contributor.otherMahidol Universityen_US
dc.contributor.otherSrinakharinwirot Universityen_US
dc.date.accessioned2018-11-09T01:51:13Z
dc.date.available2018-11-09T01:51:13Z
dc.date.issued2014-08-11en_US
dc.description.abstractA piezoelectric-based immunosensor was developed for high density lipoprotein particle (HDL-P) measurement. Monoclonal anti-human apolipoprotein A1 antibody was used as a specific binding molecule for the major apolipoprotein of HDL-P. This sensing element was fabricated by immobilizing the anti-human apolipoprotein A1 on a 12 MHz AT-cut quartz crystal via a 3-mercaptopropionic acid (MPA) self-assembled monolayer. The frequency shift from the mass change of the antigen-antibody binding refers to the amount of HDL-P. The optimal antibody immobilization was performed to achieve the maximum potential of the antibody. The appropriate quantity and immobilization time of the antibody were 0.1 mg ml−1and 90 minutes, respectively. The immobilized antibody in the HDL-P immunosensor accomplished perfect binding with HDL-P within 60 minutes. The dose-response curve for HDL-P showed a linear response from 0.21 to 2.50 mg protein per ml equivalent to 0.40 × 1010to 3.65 × 1010particles per μl without significant interference from other lipoproteins. The intra- and inter-assay imprecision (CV) were 7.8 and 18.5%, respectively. The analytical accuracy of this measurement was 96.29-96.31%. The HDL-P concentration obtained from the sensor revealed a 2.05 mg protein per ml with 0.26 mg protein per ml of expanded uncertainty at the 95% confidence level. This immunosensor gave an assay result which correlated with the homogeneous enzymatic colorimetric assay (R2= 0.902). © 2014 the Partner Organisations.en_US
dc.identifier.citationAnalyst. Vol.139, No.18 (2014), 4586-4592en_US
dc.identifier.doi10.1039/c4an00601aen_US
dc.identifier.issn13645528en_US
dc.identifier.issn00032654en_US
dc.identifier.other2-s2.0-84906092923en_US
dc.identifier.urihttps://repository.li.mahidol.ac.th/handle/20.500.14594/33232
dc.rightsMahidol Universityen_US
dc.rights.holderSCOPUSen_US
dc.source.urihttps://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84906092923&origin=inwarden_US
dc.subjectBiochemistry, Genetics and Molecular Biologyen_US
dc.subjectChemistryen_US
dc.subjectEnvironmental Scienceen_US
dc.titleA piezoelectric-based immunosensor for high density lipoprotein particle measurementen_US
dc.typeArticleen_US
dspace.entity.typePublication
mu.datasource.scopushttps://www.scopus.com/inward/record.uri?partnerID=HzOxMe3b&scp=84906092923&origin=inwarden_US
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