Antioxidant activity of Bifidobacterium animalis MSMC83 and its application in set-style probiotic yoghurt

Abstract

This study aims to investigate the antioxidative activities of probiotics derived from healthy human infants and to develop probiotic yoghurt. Viable cells, cell free supernatant and intracellular cell free extract of MSMC83 exhibited antioxidant activities. MSMC83 was assigned to Bifidobacterium animalis with 99% similarly based on 16 S rRNA gene sequence analysis. B. animalis MSMC83 showed high survival rate in simulated gastrointestinal conditions, a substantial adherence to Caco-2 cells and production of bile salt hydrolase. Set-style yoghurt fermented with B. animalis MSMC83 and yoghurt starter cultures was produced. Microbiological and physicochemical properties of yoghurt were investigated during refrigerated storage. Fermentation time of probiotic yoghurt was 4.5 h compared to 6.0 h found in regular yoghurt. The viable count of B. animalis remained at 8.53 log CFU/g after 4-week storage at 4 °C. The probiotic yoghurt possessed firmer texture and lower syneresis compared to the yoghurt fermented with starter cultures. Our results support the use of B. animalis MSMC83 as probiotic culture in the development of yoghurt with antioxidative property.