Publication: Production of zika virus virus-like particles
Issued Date
2021-01-01
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ISSN
19406029
10643745
10643745
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2-s2.0-85091472564
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Mahidol University
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SCOPUS
Bibliographic Citation
Methods in Molecular Biology. Vol.2183, (2021), 183-203
Suggested Citation
Atichat Kuadkitkan, Suwipa Ramphan, Suchin Worawichawong, Wannapa Sornjai, Nitwara Wikan, Duncan R. Smith Production of zika virus virus-like particles. Methods in Molecular Biology. Vol.2183, (2021), 183-203. doi:10.1007/978-1-0716-0795-4_10 Retrieved from: https://repository.li.mahidol.ac.th/handle/123456789/76429
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Title
Production of zika virus virus-like particles
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Abstract
Zika virus (ZIKV) is a mosquito-transmitted virus that has caused major outbreaks of disease around the world over the last few years. The infectious ZIKV consists of a structural protein outer shell surrounding a nucleocapsid. Virus-like particles (VLP) consist of the outer structural protein shell, but without the nucleocapsid, and are hence noninfectious. VLP, however, are structurally equivalent to the native virus and thus present a similar antigenic profile. These properties make them good candidates for vaccine development. ZIKV VLP can be generated on a laboratory scale by cloning the relevant structural proteins into a eukaryotic expression vector and transfecting the construct into mammalian cells. The secreted VLP can be harvested from the culture medium and purified by sucrose cushion ultracentrifugation. Validation of the VLP is achieved through western blotting and electron microscopy.